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merck millipore,默克密理博,46-645MAG,MILLIPLEX MAP Phospho Akt/PKB (assay buffer 1) (Thr308) Magnetic Bead MAPmate - Cell Signaling Single Plex
产品名称：MILLIPLEX MAP Phospho Akt/PKB (assay buffer 1) (Thr308) Magnetic Bead MAPmate - Cell Signaling Single Plex
产品型号：46-645MAG
This MILLIPLEX MAP Phospho Akt/PKB (assay buffer 1) (Thr308) Magnetic Bead MAPmate Cell Signaling Single Plexhas the analyte Akt/PKB (Thr308).
merck millipore,默克密理博,46-645MAG,MILLIPLEX MAP Phospho Akt/PKB (assay buffer 1) (Thr308) Magnetic Bead MAPmate - Cell Signaling Single Plex
- 产品介绍
- merck millipore,默克密理博,46-645MAG,MILLIPLEX MAP Phospho Akt/PKB (assay buffer 1) (Thr308) Magnetic Bead MAPmate - Cell Signaling Single Plex
重要规格表
可用的分析物 品种反应性 主要应用 检测方法 Akt/PKB (Thr308) R, H, M Mplex Luminex xMAP 描述 产品目录编号 46-645MAG 商名 - MILLIPLEX
描述 MILLIPLEX MAP Phospho Akt/PKB (assay buffer 1) (Thr308) Magnetic Bead MAPmate - Cell Signaling Single Plex 背景信息 Akt
• Also known as Protein Kinase B or PKB
• Involved in the PI3K/Akt/mTOR pathway
• Functions in processes such as cellular (glucose) metabolism, proliferation, transcription, apoptosis, cell cycle
• Associated with cancer and tumors such as neuroblastoma
• It is a target for inhibitor/drug development for cancer产品信息 检测方法 Luminex xMAP 配置 Single Plex 板面类型 Cell Signaling 应用 应用 This MILLIPLEX MAP Phospho Akt/PKB (assay buffer 1) (Thr308) Magnetic Bead MAPmate Cell Signaling Single Plexhas the analyte Akt/PKB (Thr308). 主要应用 - Multiplexing
应用说明 • An overnight (4°C) incubation is recommended for best results
• These assays require 25 µL diluted cell lysate per well
• Please consult the buffer compatibility chart or protocol for appropriate buffer choice
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg)生物信息 品种反应性 RatHumanMouse 可用的分析物 - Akt/PKB (Thr308)
Entrez基因编号 - NM_005163.2
- NM_001014431.1
- NM_001014432.1
Entrez基因汇总 The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. 基因符号 - RAC
- AKT1
- CAKT
- PKB
UniProt编号 - P31749
UniProt汇总 FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended storage for kit components is 2 - 8°C 包装信息 数量 96-well plate
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