merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB5428,Anti-Retinal Pigment Epithelium 65 Antibody

  • merck millipore,默克密理博,MAB5428,Anti-Retinal Pigment Epithelium 65 Antibody

    产品名称：Anti-Retinal Pigment Epithelium 65 Antibody
    产品型号：MAB5428
    Anti-Retinal Pigment Epithelium 65 Antibody detects level of Retinal Pigment Epithelium 65 & has been published & validated for use in ELISA, IH, IP & WB.

    merck millipore,默克密理博,MAB5428,Anti-Retinal Pigment Epithelium 65 Antibody

  • 产品介绍
  • merck millipore,默克密理博,MAB5428,Anti-Retinal Pigment Epithelium 65 Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Xn, B, H, MWB, ELISA, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB5428
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Retinal Pigment Epithelium 65 Antibody
    Alternate Names
    • RPE65
    产品信息
    格式 Purified
    控制
    • Eye
    演示 Purified immunoglobulin. Liquid in PBS. Contains no preservative.
    应用
    应用 Anti-Retinal Pigment Epithelium 65 Antibody detects level of Retinal Pigment Epithelium 65 & has been published & validated for use in ELISA, IH, IP & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunohistochemistry
    应用说明 Western blot: 1:5,000-1:20,000 on bovine RPE membranes using ECL. Suggested dilution buffer is TBS containing 10% calf serum, 0.25% T-20, 1M D-glucose with 10% glycerol. Suggested blocking buffer is TBS containing 2% BSA and 0.5% Tween 20. Preferred gel percentage is 10%.

    Immunohistochemistry on frozen tissue sections: 1:250-1:500

    Immunoprecipitation: 20 μg of antibody in a reaction volume of 500 μL.

    Immunoaffinity purification

    ELISA

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Bovine RPE microsomal membranes.
    克隆 401.8B11.3D9
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Reacts with Retinal Pigment Epithelium 65 (RPE65). On bovine RPE membranes the antibody recognizes a protein with a molecular weight of ~65 kDa.
    同种型 IgG
    品种反应性 XenopusBovineHumanMouse
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_000329.2
    Entrez基因汇总 This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.
    基因符号
    • sRPE65
    • RPE65
    • mRPE65
    • rd12
    • LCA2
    • p63
    • RP20
    纯化方法 Protein A purfied
    UniProt编号
    • Q16518
    UniProt汇总 FUNCTION: SwissProt: Q16518 # Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis- retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (By similarity).
    SIZE: 533 amino acids; 60948 Da
    SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane; Lipid-anchor (By similarity). Note=Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated (By similarity).
    TISSUE SPECIFICITY: Retinal pigment epithelium specific.
    PTM: Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all- trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A) (By similarity).
    DISEASE: SwissProt: Q16518 # Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children. & Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:180069]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant.
    SIMILARITY: SwissProt: Q16518 ## Belongs to the carotenoid oxygenase family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB5428,Anti-Retinal Pigment Epithelium 65 Antibody

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