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merck millipore,默克密理博,MAB3747,Anti-Microphthalmia (Mi) Antibody, clone C5
产品名称：Anti-Microphthalmia (Mi) Antibody, clone C5
产品型号：MAB3747
Anti-Microphthalmia (Mi) Antibody, clone C5 is a Mouse Monoclonal Antibody for detection of Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor & has been tested in EMSA, More>>
merck millipore,默克密理博,MAB3747,Anti-Microphthalmia (Mi) Antibody, clone C5
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB, EMSA, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB3747 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Microphthalmia (Mi) Antibody, clone C5 背景信息 MiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3' half of Mi including most of the b-HLH-ZIP region. Osteoclasts from these animals lack Mi protein in contrast to wild-type rat, mouse, and human osteoclasts. 产品信息 格式 Purified 控制 - 501 Mel human melanoma cells, wild-type human, rat, mouse osteoclast cells.
演示 Purified mouse monoclonal IgG1 in phosphate buffered saline with 0.08% sodium azide. 应用 应用 Anti-Microphthalmia (Mi) Antibody, clone C5 is a Mouse Monoclonal Antibody for detection of Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor & has been tested in EMSA, IHC, IHC(P), IP & WB. 主要应用 - Western Blotting
- Electrophoretic Mobility Shift Assay
- Immunohistochemistry
应用说明 Immunoprecipitation:
A previous lot of this antibody was used in IP. (2 µg/mg of protein lysate)
Gel supershift assays:
A previous lot of this antibody was used in EMSA.
Immunohistochemistry(paraffin):
A previous lot of this antibody was used in IH on frozen and formalin/paraffin tissue sections.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells. 克隆 C5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 In Western blotting, it recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi). There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells andheart. It reacts with both melanocytic as well as the nonmelanocytic isoforms of Mi. This Ab does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. 同种型 IgG1 品种反应性 MouseHumanRat Species Reactivity Note Expected to react with human and rat based on sequence homology. 抗体类型 Monoclonal Antibody 基因符号 - MAA
- LZMS
- MCOPS1
纯化方法 Protein A purfied 产品使用声明 质量保证 Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Mi on 10 μg of Mouse Brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.包装信息 数量 100 µg
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