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merck millipore,默克密理博,MAB2019Z,Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free
产品名称：Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free
产品型号：MAB2019Z
Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free is an antibody against Integrin αVβ5 for use in ELISA, FC, IP & IC.
merck millipore,默克密理博,MAB2019Z,Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, ELISA, FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB2019Z 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free Alternate Names - MAB2019
产品信息 格式 Purified 演示 Purified immunoglobulin. Liquid at 1 mg/mL in PBS, containing no preservatives. 应用 应用 Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free is an antibody against Integrin αVβ5 for use in ELISA, FC, IP & IC. 主要应用 - Immunocytochemistry
- ELISA
- Flow Cytometry
应用说明 Immunoprecipitation
Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X10E5 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.
Immunocytochemistry: 15μg/mL dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation with MAB2019Z antibody for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.
ELISA
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3. 克隆 15F11 宿主 Mouse 特异性 Reactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. The antibody binds to the alphaVbeta5 complex. The monoclonal antibody MAB2019Z has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding of the antibody to alphaVbeta5. Monoclonal antibody MAB2019Z does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor. 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002210.2
Entrez基因汇总 ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. 基因符号 - VNRA
- ITGAV
- DKFZp686A08142
- MSK8
- CD51
UniProt编号 - P06756
UniProt汇总 FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1048 amino acids; 116038 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,07-408,Anti-Smad2/3 Antibody
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