merck millipore,默克密理博,MAB1976B,Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated

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merck millipore,默克密理博,MAB1976B,Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated
产品名称：Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated
产品型号：MAB1976B
Detect Integrin αVβ3 using this Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated validated for use in FC, IH & IP.

merck millipore,默克密理博,MAB1976B,Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated

产品介绍

merck millipore,默克密理博,MAB1976B,Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Rb, Av, B, Ca, Ch, H, Mk, Po	IHC, FC	M	Biotin	Monoclonal Antibody

描述
产品目录编号	MAB1976B
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated
Alternate Names	CD51/CD61 Vitronectin Receptor
背景信息	The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors.

产品信息
格式	Biotin
控制	Chen, 1995). POSITIVE CONTROL: Human myeloma cell lines M21 and MoalphaV (Chen, 1995). NEGATIVE CONTROL: Mo cells (this cell line was derived from M21 but does not express integrin alphaV
演示	Liquid in 0.01M PBS (pH 7.1), 0.1% sodium azide with 15 mg/mL BSA added as a stabilizer.

应用
应用	Detect Integrin αVβ3 using this Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated validated for use in FC, IH & IP.
主要应用	Immunohistochemistry Flow Cytometry
不建议的应用	Immunohistochemistry (Paraffin)
应用说明	Immunoprecipitation: specifically immunoprecipitates the integrin alphaVbeta3 complex which, when resolved by SDS-PAGE under reducing conditions, results in an approximately 130 kDa band corresponding to the alphaV subunit and a 105 kDa band corresponding to the beta3 subunit (Cheresh, 1987). Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections. Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 μg/mL. Optimal working dilutions and protocols must be determined by end user.

生物信息
免疫原品种	Purified adhesion receptor from the human melanoma cell line M21 (Cheresh, 1987).
克隆	LM609
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Reacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, 1987). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, 1994). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
同种型	IgG1
品种反应性	RabbitAvianBovineCanineChickenHumanMonkeyPig
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_002210.2
Entrez基因汇总	ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
基因符号	VNRA ITGAV DKFZp686A08142 MSK8 CD51
非反应性品种	Rat Mouse
纯化方法	Protein A purfied
UniProt编号	P06756
UniProt汇总	FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. SIZE: 1048 amino acids; 116038 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain at 2–8°C in undiluted aliquots for up to 6 months.

包装信息
数量	100 µg

merck millipore,默克密理博,MAB1976B,Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated

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