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品种反应性 主要应用 宿主 格式 抗体类型 H ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB1952P Replaces MAB1952 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin α3 Antibody, clone P1B5 Alternate Names - CD49c
背景信息 Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys. 产品信息 格式 Purified 控制 - Human foetal foreskin fibroblasts (HFFF), neuroblastomas
演示 Purified in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl containing with 0.05% NaN3. 应用 应用 Detect Integrin α3 using this Anti-Integrin α3 Antibody, clone P1B5 validated for use in IC, IH(P). 主要应用 - Immunocytochemistry
应用说明 Suitable for use in attachment inhibition assays using fibroblasts, most epithelial cells, activated lymphocytes on laminin, collagen and fibronectin.
Immunohistochemistry: 1:50 - 1:200 dilution on formalin-fixed paraffin-embedded human colorectal tubular adenoma tissue; requires pretreatment with citrate buffer, pH 6.0, using high heat eptiope retrieval techniques
Inhibits Activity/Function: It has been shown by independent labs that clone P1B5 inhibits adhesion of the alpha3 integrin subunit. (Miles, et al, 1995, Wayner, et al, 1988).
Optimal working dilutions must be determined by end user.生物信息 表位 Cell-binding domain 克隆 P1B5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with human integrin alpha3. 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005501.1
- NM_002204.1
Entrez基因汇总 ITGA3 encodes the integrin alpha 3 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 3 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that interacts with many extracellular-matrix proteins. The alpha 3 beta 1 integrin is known variously as: very late (activation) antigen 3 ('VLA-3'), very common antigen 2 ('VCA-2'), extracellular matrix receptor 1 ('ECMR1'), and galactoprotein b3 ('GAPB3'). 基因符号 - CD49C
- ITGA3
- GAP-B3
- CD49c
- FLJ34704
- MSK18
- VLA3a
- VL3A
- VCA-2
- FLJ34631
- FRP-2
- GAPB3
纯化方法 Protein G chromatography UniProt编号 - P26006
UniProt汇总 FUNCTION: SwissProt: P26006 # Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha- 3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
SIZE: 1066 amino acids; 118698 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-3 associates with beta-1. Interacts with HPS5.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
PTM: Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12- myristate 13-acetate stimulation.
SIMILARITY: SwissProt: P26006 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 1 year from date of receipt. 包装信息 数量 100 µg
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