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merck millipore,默克密理博,MAB1273,Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1
产品名称：Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1
产品型号：MAB1273
Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 is an antibody against Mitochondria for use in IF, IH, IH(P) & IP.
merck millipore,默克密理博,MAB1273,Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1
- 产品介绍
- merck millipore,默克密理博,MAB1273,Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB1273 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 背景信息 This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions. 产品信息 格式 Purified 控制 - COS7 cells.
演示 Purified mouse monoclonal IgG1 in buffer containing liquid in PBS. Contains no preservative. Concentrated hybridoma supernatant. 应用 应用 Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 is an antibody against Mitochondria for use in IF, IH, IH(P) & IP. 主要应用 - Immunohistochemistry
应用说明 Immunohistochemistry:
1:100 dilutoin from a previous lot was used. Works best with organic fixatives (acetone, methanol, etc). 2% PFA for 10-15 min can be used but stronger use of formalin or 4% PFA limits staining.
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads.
Immunohistochemistry in paraffin embedded tissues:
Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads.
Indirect immunofluorescence:
1:10-1:50 dilution from a previous lot was used, 30 - 100 μL per slide well.
MAB1273 recognizes a 65 kD protein by immunoprecipitation.
Optimal working dilutions and protocols must be determined by the end user.生物信息 表位 surface of intact mitochondria 克隆 113-1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Gives mitochondrial staining on all human cell types and does not cross react with rat and mouse tissue. The staining pattern is best described as a spaghetti-like staining pattern in fibroblasts and a perinuclear large speckled pattern in lymphonoid cells.
MAB1273 recognizes a 65 kD protein by immunoprecipitation. Antibody produces the stringy, "spaghetti-like" staining patten in the cytoplasm of human cells.同种型 IgG1 品种反应性 Human Species Reactivity Note Human. Not rat or mouse. Reactivity with other species has not been determined 抗体类型 Monoclonal Antibody 纯化方法 Ammonium sulfate precipitation 产品使用声明 质量保证 Routinely evaluated by immunohistochemistry on heart ventricle cells.
Immunohistochemistry(paraffin) Analysis:
Mitochondria (cat. # MAB1273) staining on heart ventricle cells. Tissue pretreated with EDTA, pH 8.0This lot of antibody was diluted to 1:80, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at -20°C in undiluted aliquots from date of receipt. 包装信息 数量 100 µL
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