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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC, FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB1219 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-CD14 Antibody, clone 2D-15C 概述 BIOCHEMISTRY: p I = 5.8 Alternate Names - LPS Receptor
产品信息 格式 Purified 演示 The purified antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry. 应用 应用 Anti-CD14 Antibody, clone 2D-15C detects level of CD14 & has been published & validated for use in FC, IH. 主要应用 - Immunohistochemistry
- Flow Cytometry
应用说明 This antibody is an important general cell marker for mononuclear phagocytes in normal samples and a variety of disease states including a proportion of cases of myeloid leukemia. It is suitable for flow cytometry, and immunoperoxidase staining on frozen tissue sections.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:25
Dilute with isotonic buffer. Use 50 μl per 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems.生物信息 免疫原品种 Human peripheral blood mononuclear cells. 克隆 2D-15C 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody belongs to CD14 (assigned by the Third International Workshop on Leucocyte Differentiation Antigens, Oxford, 1986; McMichael et al. Eds., 1986). and reacts with 50 to 55kD protein. It will detect blood monocytes, Kupffer cells, red pulp macrophages, dendritic cells and also "epithelioid" or giant cells within granulomatous tissue (Hancock et al., 1983), foam cells in renal interstitium (Nolasco et al., 1984) and placental dendritic cells and macrophages (Barnett et al., 1984; Brooks et al., 1983; Polli et al., 1984; Hopper et al., 1986). Monocytes react with this antibody in the early stages of differentiation, promonocytes and monoblasts do not. Activity is not lost on monocyte/macrophage activation (Hancock et al., 1983).
Cell reactivity
Reacts with peripheral blood monocytes and weakly with granulocytes. Negative to platelets, erythrocytes and lymphocytes. Sometimes positive for chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). Negative to B-cell chronic lymphocytic leukemia (B-CLL), T-cell chronic lymphocytic leukemia (T-CLL) and common acute lymphoblastic leukemia (CALL).同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001040021.1
- NM_000591.2
Entrez基因汇总 CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. 基因符号 - CD14
UniProt编号 - P08571
UniProt汇总 FUNCTION: SwissProt: P08571 # Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
SIZE: 375 amino acids; 40076 Da
SUBUNIT: Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
TISSUE SPECIFICITY: Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
SIMILARITY: SwissProt: P08571 ## Contains 11 LRR (leucine-rich) repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.包装信息 数量 100 µg
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