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merck millipore,默克密理博,HTS155M,ChemiSCREEN™ Membrane Preparation Recombinant Human MC5 Melanocortin Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human MC5 Melanocortin Receptor
产品型号：HTS155M
Human MC5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS155M,ChemiSCREEN™ Membrane Preparation Recombinant Human MC5 Melanocortin Receptor
- 产品介绍
- merck millipore,默克密理博,HTS155M,ChemiSCREEN™ Membrane Preparation Recombinant Human MC5 Melanocortin Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 MC5 描述 产品目录编号 HTS155M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human MC5 Melanocortin Receptor 概述 Full-length human MC5R cDNA encoding MC5 背景信息 The melanocortin system consists of five seven-transmembrane spanning G-protein coupled receptors (MC1-MC5). Among the five members of the melanocortin receptor family, MC2 and MC5 are expressed in peripheral tissues. The MC2 receptor (ACTH receptor) is almost exclusively expressed in the adrenal cortex whereas MC5 is expressed in several organs including the adrenal cortex. Studies have shown that targeted disruption of the MC5R gene produced mice with a severe defect in water repulsion and thermoregulation caused by decreased production of sebaceous lipids (Chen et al., 1997). Data show a requirement for the MC5 in multiple exocrine glands for the production of numerous products, indicative of a coordinated system for regulation of exocrine gland function by melanocortin peptides. (Entwistle et al.,1990). Millipore's MC5 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of MC5. The membrane preparations exhibit a Kd of 1.3nM for [125I]-[Nle4, D-Phe7]-α-MSH (NDP-αMSH). With 0.3 nM [125I]-NDP-αMSH, 10µg/well MC5 Membrane Prep typically yields greater than 50-fold signal-to-background ratio. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80°C 应用 应用 Human MC5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_005913.1
基因符号 - MC-2
- MC5R
- MC5-R
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 MC5 靶子系列 Melanocortin UniProt编号 - P33032
UniProt汇总 FUNCTION: SwissProt: P33032 # Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins.
SIZE: 325 amino acids; 36601 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in the brain but not in the melanoma cells.
SIMILARITY: SwissProt: P33032 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min,, then washed with 50mM HEPES, pH 7.4, 500mM NaCl. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, filtered and stored at 4°C.
Radioligand: [125I]-NDP-αMSH (Perkin Elmer#: NEX-352)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 50-fold signal:background with 125I labeled NDP-αMSH at 0.3 nM产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with MC5 Receptor membrane prep. 10 µg/well Signal:Background 147.3 Specific Binding (cpm) 15208.7
SPECIFICATIONS: 1 unit = 10 µg
Bmax for [125I]-NDP-αMSH binding: 4.3 pmol/mg protein
Kd for [125I]-NDP-αMSH binding: ~1.3 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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