- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,HTS134M,ChemiSCREEN™ Membrane Preparation Recombinant Human GIP Glucagon Family Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human GIP Glucagon Family Receptor
产品型号：HTS134M
Human GIP GPCR membrane preparation for Radioligand binding Assays.
merck millipore,默克密理博,HTS134M,ChemiSCREEN™ Membrane Preparation Recombinant Human GIP Glucagon Family Receptor
- 产品介绍
- merck millipore,默克密理博,HTS134M,ChemiSCREEN™ Membrane Preparation Recombinant Human GIP Glucagon Family Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay Human Chem-9 GIP 描述 产品目录编号 HTS134M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human GIP Glucagon Family Receptor 概述 Full-length human GIPR cDNA encoding GIP Receptor Alternate Names - GIPR, Gastric inhibitory polypeptide
背景信息 Gastric inhibitory polypeptide (GIP), also known as glucose-dependent insulinotropic polypeptide, is a 42 amino acid peptide that is secreted from the duodenum and proximal jejunum after food intake. The receptor for GIP is a family 2 GPCR that is most closely related to the glucagon and GLP-1 receptors, and like these receptors it couples to Gs to stimulate intracellular cAMP (Mayo et al., 2003). The GIP receptor is most highly expressed in pancreas, and the primary physiological function of GIP is to potentiate glucose-mediated secretion of insulin (Yip et al., 1999). The GIP receptor is expressed in many other tissues, and accordingly GIP also has extrapancreatic activities. GIP secreted after meal ingestion stimulates bone formation, and mice lacking the GIP receptor display features of osteoporosis (Tsukiyama et al., 2006). In addition, GIP increases insulin sensitivity in adipose tissue. Since patients with type 2 diabetes display decreased responsiveness to GIP, a defect in GIP receptor expression and/or signaling may lead to β-cell dysfunction and type 2 diabetes (Meier and Nauck, 2004). Millipore's GIP membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at GIP Receptor. The membrane preparations exhibit a Kd of 0.44 nM for [125I]-GIP. With 0.2 nM [125I]-GIP, 10 µg/well GIP Receptor Membrane Prep typically yields greater than 8-fold signal-to-background ratio. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human GIP GPCR membrane preparation for Radioligand binding Assays. 应用说明 Radioligand binding assay 生物信息 品种 Human 品种反应性 Human Entrez基因编号 - NM_000164.2
Entrez基因汇总 Gastric inhibitory polypeptide (GIP; MIM 137240), also called glucose-dependent insulinotropic polypeptide, is a 42-amino acid polypeptide synthesized by K cells of the duodenum and small intestine. It was originally identified as an activity in gut extracts that inhibited gastric acid secretion and gastrin release, but subsequently was demonstrated to stimulate insulin release potently in the presence of elevated glucose. The insulinotropic effect on pancreatic islet beta-cells was then recognized to be the principal physiologic action of GIP. Together with glucagon-like peptide-1, GIP is largely responsible for the secretion of insulin after eating. It is involved in several other facets of the anabolic response.[supplied by OMIM] 基因符号 - GIP-R
- GIPR
- MGC126722
GPCR类别 B 宿主细胞 Chem-9 蛋白靶 GIP 靶子系列 Glucagon UniProt编号 - P48546
UniProt汇总 FUNCTION: SwissProt: P48546 # This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 466 amino acids; 53157 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P48546 ## Belongs to the G-protein coupled receptor 2 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] GIP (Perkin Elmer#: NEX-402)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 8-fold signal:background with 125I-labeled GIP at 0.2 nM产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with GIP Receptor membrane prep and unlabeled GIP.
10 µg/well Signal:Background 12.7 Specific Binding (cpm) 11304
SPECIFICATIONS: 1 unit = 10 µg
Bmax for [125I] GIP binding: 3.7 pmol/mg protein
Kd for [125I] GIP binding: ~0.44 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at –80°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,2090-OP,OmniPur Agarose, High Gel Strength - CAS 9012-36-6 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,ST1631,Anti-BMP7 Mouse mAb (M1-F8)
相关产品
merck millipore,默克密理博,NA13,Anti-Replication Protein A (Ab-1) Mouse mAb (RPA70-9)
merck millipore,默克密理博,GF317,TGF-β-III Protein, Human Recombinant Animal Free
merck millipore,默克密理博,SF-2940,CYP3A4; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,AB1128,Anti-Respiratory Syncytial Virus Antibody
merck millipore,默克密理博,2090-OP,OmniPur Agarose, High Gel Strength - CAS 9012-36-6 - Calbiochem
merck millipore,默克密理博,ST1631,Anti-BMP7 Mouse mAb (M1-F8)
%5BABE438_IHC(P)-ALL%5D.jpg "merck millipore,默克密理博,ABE438,Anti-Apobec1 Antibody")
merck millipore,默克密理博,ABE438,Anti-Apobec1 Antibody
merck millipore,默克密理博,341006,EDAC, Hydrochloride
merck millipore,默克密理博,SCR108,Human Mesenchymal Stem Cell Kit - Derived from Bone-Marrow