- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,HTS078M,ChemiSCREEN™ Human Recombinant S1P2 Lysophospholipid Receptor Membrane Preparation
产品名称：ChemiSCREEN™ Human Recombinant S1P2 Lysophospholipid Receptor Membrane Preparation
产品型号：HTS078M
Human S1P2 / EDG5 GPCR membrane preparation for GTPγS Binding & Radioligand Binding Assays. Less<<
merck millipore,默克密理博,HTS078M,ChemiSCREEN™ Human Recombinant S1P2 Lysophospholipid Receptor Membrane Preparation
- 产品介绍
- merck millipore,默克密理博,HTS078M,ChemiSCREEN™ Human Recombinant S1P2 Lysophospholipid Receptor Membrane Preparation
重要规格表
应用说明 品种 宿主细胞 蛋白靶 GTPγS Binding and Radioligand Binding Assay. Human Chem-1 S1P2 / EDG5 描述 产品目录编号 HTS078M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Human Recombinant S1P2 Lysophospholipid Receptor Membrane Preparation 概述 human EDG-5 cDNA encoding S1P2 背景信息 Sphingosine 1-phosphate (S1P) is a bioactive lipid that binds to and activates a family of GPCRs, S1P1-5 (also known as EDG receptors). Interactions between S1P and its receptors mediate cytoskeletal rearrangement and cell migration, with functional consequences in angiogenesis, lymphocyte trafficking, and smooth muscle development (Anliker and Chun, 2004). S1P1 (Edg-1) signals exclusively through Gi, whereas S1P2 (Edg-5) and S1P3 (Edg-3) activate Gi, Gq and G12/13 (Windh et al., 1999). Although S1P1 and S1P3 promote cell migration, S1P2 inhibits cell migration in several cell types; these opposing functions appear to result from differences in the ability of each receptor to activate Gi (Arikawa et al., 2003; Sugimoto et al., 2003; Goparaju et al., 2005). Studies with knockout mice indicate that S1P2 and S1P3 have redundant functions in maintaining vascular integrity during embryonic development (Kono et al., 2004). Chemicon's S1P2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of S1P2 interactions with its ligands. The cell line exhibits EC50 of 7.2nM for S1P in a GTPγS binding assay. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol with no preservatives. Packaging method: Membrane protein was adjusted to 1 mg/ml in packaging buffer, rapidly frozen, and stored at -80°C. 应用 应用 Human S1P2 / EDG5 GPCR membrane preparation for GTPγS Binding & Radioligand Binding Assays. 应用说明 GTPγS Binding and Radioligand Binding Assay. 生物信息 浓缩 1 mg/mL 品种 Human Entrez基因编号 - NM_004230.3
Entrez基因汇总 This gene encodes a member of the G protein-coupled receptors, as well as the EDG family of proteins. This protein participates in sphingosine 1-phosphate-induced cell proliferation, survival, and transcriptional activation 基因符号 - S1PR2
- EDG5
- S1P2
- Gpcr13
- AGR16
- EDG-5
- LPB2
- H218
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 S1P2 / EDG5 靶子系列 Lysophospholipid UniProt编号 - O95136
UniProt汇总 FUNCTION: SwissProt: O95136 # Receptor for the lysosphingolipid sphingosine 1- phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
SIZE: 353 amino acids; 38867 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: O95136 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.1 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled S1P is added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to a GF/B filter plate (Millipore MAHF B1H) previously prewetted with water, and washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4. The plate is dried and counted.
One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific S1P -stimulated [35S]-GTPγS binding.
The S1P2 membrane preparation is expected to be functional in a radioligand binding assay; however, the end user will need to determine the optimal radiolabeled ligand for use with this product.产品使用声明 质量保证 EC50 in GTPγS binding assay by S1P: ~ 7.2nM 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,SF-1309,MSI1; Ms, Cyanine 5 RNA Detection Probe,SmartFlare
下一件merck millipore产品：merck millipore,默克密理博,14-173,MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg
相关产品
merck millipore,默克密理博,36-013,Anti-phospho-MKK7/SKK4 (Thr275) Antibody
merck millipore,默克密理博,100081,14-3-3 Antagonist I, 2-5 - Calbiochem
merck millipore,默克密理博,MAB8614,Anti-Varicella-Zoster Virus Antibody, nucleocapsid protein
merck millipore,默克密理博,NE1042,Anti-INHBA Rabbit pAb
merck millipore,默克密理博,SF-1309,MSI1; Ms, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,14-173,MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg
merck millipore,默克密理博,SCGP00525,Steriflip-GP，0.22 µm，改良聚醚砜，已经 γ 射线照射
merck millipore,默克密理博,MAB8910-KC,Mouse Anti-Measles (Matrix Protein), clone CV8, CV9 (1mg) KC
merck millipore,默克密理博,07-2149,Anti-Rho GTPase-activating protein 23 Antibody
