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merck millipore,默克密理博,HTS057M,ChemiScreen™ Recombinant Human PRP/GPR10 Prolactin-Releasing Peptide Receptor Membrane Preparation
产品名称：ChemiScreen™ Recombinant Human PRP/GPR10 Prolactin-Releasing Peptide Receptor Membrane Preparation
产品型号：HTS057M
Human PRP / PrRP / GPR10 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS057M,ChemiScreen™ Recombinant Human PRP/GPR10 Prolactin-Releasing Peptide Receptor Membrane Preparation
- 产品介绍
- merck millipore,默克密理博,HTS057M,ChemiScreen™ Recombinant Human PRP/GPR10 Prolactin-Releasing Peptide Receptor Membrane Preparation
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPγS binding. Human Chem-1 PRP / PrRP / GPR10 描述 产品目录编号 HTS057M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ Recombinant Human PRP/GPR10 Prolactin-Releasing Peptide Receptor Membrane Preparation 概述 Human Full-length human GPR10 cDNA encoding PRP 背景信息 PRP, also known as GPR10 or hGR3, is a Gq-coupled receptor for prolactin-releasing peptide that is expressed in the pituitary (Hinuma et al., 1998). Genetic studies in rodents indicate that lack of GPR10 leads to hyperphagia, obesity and dyslipidemia (Gu et al., 2004; Watanabe et al., 2005). In humans, genetic variations in GPR10 are associated with lowered blood pressure (Bhattacharyya et al., 2003). Chemicon's GPR10/PRP membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of GPR10/PRP interactions with prolactin-releasing peptide. The membrane preparations exhibit a Kd of 0.59-0.66 nM for [125I]-PRP-20. With 5 µg/well PRP Membrane Prep and 0.25 nM [125I]-PRP-20, a greater than 30-fold signal-to-background ratio was obtained. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 30-fold signal:background with 125I-labeled PRP-20 at 0.25 nM.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human PRP / PrRP / GPR10 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay, and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_004248.2
Entrez基因汇总 PRLHR is a 7-transmembrane domain receptor for prolactin-releasing hormone (PRLH; MIM 602663) that is highly expressed in anterior pituitary (Ozawa et al., 2002 [PubMed 11923475]).[supplied by OMIM] 基因符号 - MGC126541
- PRLHR
- GR3
- GPR10
- MGC126539
- PrRPR
- hGR3
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 PRP / PrRP / GPR10 靶子系列 Prolactin-releasing peptide UniProt编号 - P49683
UniProt汇总 FUNCTION: SwissProt: P49683 # Receptor for prolactin-releasing peptide (PrRP). Implicated in lactation, regulation of food intake and pain-signal processing.
SIZE: 370 amino acids; 41107 Da
SUBUNIT: Interacts through its C-terminal region with the PDZ domain-containing proteins GRIP1, GRIP2 and PICK1. Interacts with PDZ domains 4 and 5 of GRIP1 and with the PDZ domain of PICK1.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Only detected in the pituitary gland and in all cell types of pituitary adenomas.
SIMILARITY: SwissProt: P49683 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] PRP-20 (Perkin Elmer # NEX384)
Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C.产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with varying amounts of PRP membrane prep:
10 µg/well 5 µg/well Signal:Background 46.0 51.6 Specific Binding (cpm) 15756 14148
SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax: 8.9 pmol/mg
Kd: 0.62 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units 包装 1 mL, 1 mg/mL
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