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应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPgammaS binding. Human Chem-1 GnRH / LHRH 描述 产品目录编号 HTS027M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ GnRH Membrane Preparation 概述 Human Type I GnRH receptor 背景信息 Gonadotropin-releasing hormone (GnRH), also known as luteinizing hormone-releasing hormone (LHRH), regulates the reproductive hormonal cascade in vertebrates. Upon release from the hypothalamus, GnRH stimulates secretion of luteinizing hormone and follicle-stimulating hormone from the pituitary. In humans, the type I GnRH receptor is a GPCR that is unusual in the lack of a cytoplasmic C-terminal tail. Although some species contain a type II GnRH receptor, the human version of this second receptor appears to contain mutations that render it inactive. GnRH analogs (agonists) are used in low doses in a pulsatile fashion in the treatment of infertility, delayed puberty and cryptorchidism. GnRH agonists at high doses desensitize the receptor, and are used along with antagonists in the treatment of hormone-dependent diseases (Millar et al., 2004). Chemicon's cloned human type I GnRH receptor-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GnRH receptor expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists of the GnRH receptor. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.Packaging method: Membranes protein were adjusted to 2.0 mg/ml in 1 mL packaging buffer, rapidly frozen, and stored at -80oC. 应用 应用 Human GnRH / LHRH GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding. 应用说明 Radioligand binding assay, and GTPgammaS binding. 生物信息 浓缩 0.5 mg/mL 品种 Human 品种反应性 Human Entrez基因编号 - NM_001083111.1
- NM_000825.3
基因符号 - GRH
- GNRH1
- GNRH
- LHRH
- LNRH
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 GnRH / LHRH 靶子系列 GnRH UniProt编号 - P01148
UniProt汇总 FUNCTION: SwissProt: P01148 # Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.
SIZE: 92 amino acids; 10380 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01148 ## Belongs to the GnRH family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50 mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I]-[D-Trp6]-LHRH (Perkin Elmer # NEX365)
Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 3-fold signal:background with 125I-labeled [D-Trp6]-LHRH at 0.5nM.产品使用声明 质量保证 SPECIFICATIONS: Bmax: 1.0 pmol/mg
Kd: 0.3 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 assays
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