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merck millipore,默克密理博,HNCSMAG-35K,MILLIPLEX MAP Human Circadian/Stress Magnetic Bead Panel - Multiplex Assay
产品名称:MILLIPLEX MAP Human Circadian/Stress Magnetic Bead Panel - Multiplex Assay
产品型号:HNCSMAG-35K
The MILLIPLEX MAP Human Circadian/Stress Panel is a 2-plex kit containing all reagents needed for simultaneous quantification of Cortisol and/or Melatonin in serum, plasma, cerebrospin More>>
merck millipore,默克密理博,HNCSMAG-35K,MILLIPLEX MAP Human Circadian/Stress Magnetic Bead Panel - Multiplex Assay
- 产品介绍
- merck millipore,默克密理博,HNCSMAG-35K,MILLIPLEX MAP Human Circadian/Stress Magnetic Bead Panel - Multiplex Assay
重要规格表
可用的分析物 品种反应性 主要应用 检测方法 Melatonin, Cortisol H Mplex Luminex xMAP 描述 产品目录编号 HNCSMAG-35K 商名 - MILLIPLEX
描述 MILLIPLEX MAP Human Circadian/Stress Magnetic Bead Panel - Multiplex Assay 概述 The MILLIPLEX® MAP Human Circadian/Stress Magnetic Bead Panel is to be used for the simultaneous quantification of Cortisol and Melatonin. This is a configurable assay, and either or both analytes can be selected. This kit may be used for the analysis of human serum, plasma, tissue/cell lysate, culture supernatant samples, or cerebrospinal fluid (CSF).
Serum or plasma samples should be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with this kit.背景信息 The MILLIPLEX® MAP Human Circadian/Stress Magnetic Bead Panel enables you to focus on the relevance and therapeutic potential of Melatonin and Cortisol in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity. 产品信息 部件 - Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers
- Human Circadian/Stress Standard, 1 vial, lyophilized
- Human Circadian/Stress Quality Control 1, 1 vial, lyophilized
- Human Circadian/Stress Quality Control 2, 1 vial, lyophilized
- Assay Buffer, 2 bottles, 30 mL ea
- Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
- Human Circadian/Stress Primary Antibodies, 1 bottle, 3.5 mL
- Human Circadian/Stress Detection Antibodies, 1 bottle, 5.5 mL
- Human Circadian/Stress Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea
- Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
- Mixing Bottle, 1 bottle
检测方法 Luminex xMAP 配置 Design your multiplex kit by choosing analytes. 精度(%) - Intra-assay: < 15% CV
- Inter-assay: < 25% CV
板面类型 MAGNETIC Neuroscience 应用 应用 The MILLIPLEX® MAP Human Circadian/Stress Panel is a 2-plex kit containing all reagents needed for simultaneous quantification of Cortisol and/or Melatonin in serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples on Luminex® systems. 主要应用 - Multiplexing
应用说明 EMD Millipore’s MILLIPLEX® MAP Human Circadian/Stress Magnetic Bead Panel is a 2-plex kit to be used for the simultaneous quantification of cortisol and/or melatonin in serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples.
• This is a competitive assay. An overnight (16-18 hours) incubation at 2-8°C is recommended.
• This assay requires 50 μL extracted serum or plasma sample per well or 50 μL neat CSF or culture supernatant per well.
• Sample extraction protocols are required for serum/plasma samples. Other sample types (e.g. CSF, culture supernatant, saliva, etc.) do not require sample extraction.
• A 1:4 sample dilution is recommended for extracted serum/plasma samples.生物信息 特异性 There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. 品种反应性 Human 可用的分析物 - Melatonin
- Cortisol
化验面板 Everything you need in a single kit. 交叉反应性 There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. 准确度 - Spike Recovery for Cortisol and Melatonin is 120% and 129%, repsectively.
理化信息 灵敏度 - Melatonin: 4.3 pg/mL (MinDC+2SD)
- Cortisol: 686 pg/mL (MinDC+2SD)
标准曲线范围 - Melatonin: 3.4 – 2,500 pg/mL
- Cortisol: 686 – 500,000 pg/mL
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended storage for kit components is 2 - 8°C. 包装信息 数量 96-well plate 包装 96-well plate
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