merck millipore,默克密理愽
  • merck millipore,默克密理博,H0XPSMAG-16K,MILLIPLEX MAP Human Oxidative Phosphorylation (OXPHOS) Magnetic Bead Panel - Cellular Metabolism Multiplex Assay

  • merck millipore,默克密理博,H0XPSMAG-16K,MILLIPLEX MAP Human Oxidative Phosphorylation (OXPHOS) Magnetic Bead Panel - Cellular Metabolism Multiplex Assay

    产品名称：MILLIPLEX MAP Human Oxidative Phosphorylation (OXPHOS) Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
    产品型号：H0XPSMAG-16K
    The analytes available for this multiplex kit are: Complex I, Complex II, Complex III, Complex IV, Complex V, NNT.

    merck millipore,默克密理博,H0XPSMAG-16K,MILLIPLEX MAP Human Oxidative Phosphorylation (OXPHOS) Magnetic Bead Panel - Cellular Metabolism Multiplex Assay

  • 产品介绍
  • merck millipore,默克密理博,H0XPSMAG-16K,MILLIPLEX MAP Human Oxidative Phosphorylation (OXPHOS) Magnetic Bead Panel - Cellular Metabolism Multiplex Assay

    重要规格表

    可用的分析物 品种反应性 主要应用 检测方法
    NNT, Complex I, Complex II, Complex III, Complex IV, Complex VHMplexLuminex xMAP
    描述
    产品目录编号 H0XPSMAG-16K
    商名
    • MILLIPLEX
    描述 MILLIPLEX MAP Human Oxidative Phosphorylation (OXPHOS) Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
    概述 The MILLIPLEX® MAP Human Oxidative Phosphorylation (OXPHOS) Magnetic Bead Panel is available in premix format only, and may be used for the analysis of cell lysate or tissue extract.
    背景信息 Mitochondrial Oxidative Phosphorylation (OXPHOS) produces more than 95% of the conserved cellular energy in the form of ATP under normal conditions. This process involves five different protein complexes, NADH–ubiquinone oxidoreductase (Complex I), succinate ubiquinone oxidoreductase (Complex II), ubiquinone cytochrome c oxidoreductase (Complex III), cytochrome c oxidase (Complex IV) and ATP synthase (Complex V). Nicotinamide nucleotide transhydrogenase (NNT) is an integral protein of the inner mitochondrial membrane whose structure and function are closely related to OXPHOS. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Since NNT is encoded solely in the nucleus, it serves as a good internal reference for mitochondrial proteins.

    The overall process of oxidative phosphorylation is tightly controlled by transcriptional regulation at the level of DNA and RNA. Inefficient electron transfer through complexes I-IV leads to various human abnormalities. Without proper regulation, the production of toxic reactive oxygen species is known to increase. Many diverse classes of drugs inhibit OXPHOS and induce mitochondrial toxicity. The ability to monitor the expression levels of the OXPHOS complexes is a key element to the studies of many diseases and to the process of drug safety evaluation.
    产品信息
    检测方法 Luminex xMAP
    配置 Premixed
    精度（%）
    • Intra- and Inter-Assay CVs based on MFI values.
    • Inter-assay: < 15%
    • Intra-assay: < 10%
    板面类型 MAGNETIC Cellular Metabolism
    应用
    应用 The analytes available for this multiplex kit are: Complex I, Complex II, Complex III, Complex IV, Complex V, NNT.
    主要应用
    • Multiplexing
    应用说明 • Two hours incubation is recommended for best results.
    • These assays require 25 µL diluted cell lysate.
    • These kits must be run using the provided Cell/Mitochondria Lysis Buffer.
    • We recommend using 5-20 µg cell lysate/well.
    生物信息
    特异性 Each capture bead within the panel was tested for cross-reactivity to the other analytes with mass spectrometry. There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within this panel.
    品种反应性 Human
    可用的分析物
    • NNT
    • Complex I
    • Complex II
    • Complex III
    • Complex IV
    • Complex V
    化验面板 Everything you need in a single kit.
    交叉反应性 MILLIPLEX® MAP Human Oxidative Phosphorylation (OXPHOS) Magnetic Bead Panel is specific for human species.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Recommended storage for kit components is 2 - 8°C.
    包装信息
    数量 96-well plate

    merck millipore,默克密理博,H0XPSMAG-16K,MILLIPLEX MAP Human Oxidative Phosphorylation (OXPHOS) Magnetic Bead Panel - Cellular Metabolism Multiplex Assay

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