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merck millipore,默克密理博,FCMAB336PE,Milli-Mark™ Anti-PP2A-PE Antibody, C subunit, clone 1D6
产品名称：Milli-Mark™ Anti-PP2A-PE Antibody, C subunit, clone 1D6
产品型号：FCMAB336PE
Milli-Mark Anti-PP2A-PE Antibody, C subunit, clone 1D6 detects level of PP2A & has been published & validated for use in FC.
merck millipore,默克密理博,FCMAB336PE,Milli-Mark™ Anti-PP2A-PE Antibody, C subunit, clone 1D6
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC M Phycoerythrin Monoclonal Antibody 描述 产品目录编号 FCMAB336PE 商名 - Milli-Mark
描述 Milli-Mark™ Anti-PP2A-PE Antibody, C subunit, clone 1D6 Alternate Names - serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
- Replication protein C
- protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
- protein phosphatase 2, catalytic subunit, alpha isoform
背景信息 Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal downmodulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine. 产品信息 格式 Phycoerythrin 控制 - A431 cells.
演示 Purified mouse monoclonal IgG2bκ conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA. 应用 应用 Milli-Mark Anti-PP2A-PE Antibody, C subunit, clone 1D6 detects level of PP2A & has been published & validated for use in FC. 主要应用 - Flow Cytometry
生物信息 免疫原品种 A synthetic peptide corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A). 表位 a.a. 295-309 克隆 1D6 宿主 Mouse 特异性 This antibody recognizes PP2A catalytic subunit. 同种型 IgG2bκ 品种反应性 Human Species Reactivity Note Tested and passed on Human. Unconjugated (Cat. No.05-421) is known to react on Rat, Mouse, Bovine, Rabbit, Xenopus, Yeast (S. cerevisiae) 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002706
Entrez基因汇总 This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. 基因符号 - RP-C
- PPP2CA
- PP2CA
- PP2A-alpha
- PP2Ac
纯化方法 Protein A Purified UniProt编号 - P67775
UniProt汇总 FUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
SIZE: 309 amino acids; 35594 Da
SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily. ...产品使用声明 质量保证 Evaluated by flow cytometry using A431 cells. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 tests
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