merck millipore,默克密理博,CBA023,InnoZyme™ Cathepsin L Activity Kit, Fluorogenic

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merck millipore,默克密理博,CBA023,InnoZyme™ Cathepsin L Activity Kit, Fluorogenic
产品名称：InnoZyme™ Cathepsin L Activity Kit, Fluorogenic
产品型号：CBA023

merck millipore,默克密理博,CBA023,InnoZyme™ Cathepsin L Activity Kit, Fluorogenic

产品介绍

merck millipore,默克密理博,CBA023,InnoZyme™ Cathepsin L Activity Kit, Fluorogenic

重要规格表

检测方法
Fluorometric

描述
概述	A highly sensitive and selective fluorogenic assay for the detection of Cathepsin L from human and mouse samples. Cathepsin L is detected with the fluorogenic substrate Z-Phe-Arg-AMC (Exc. max: 360 nm; Emi. max: 460 nm). Interference from Cathepsin B is eliminated by the incorporation of CA 074, a specific cathepsin B inhibitor. This kit is also useful for screening cathepsin L inhibitors.
产品目录编号	CBA023
品牌系列	Calbiochem®
应用数据	Calibration curve generated using the AMC Calibration Standard included in the InnoZyme™ Cathepsin L Activity Kit. The assay was carried out as specified in the Cathepsin L Inhibitor Screening protocol.

参考
参考	Bohne, S., et al. 2004. J. Pathol.203, 528. Goulet, B., et al. 2004. Mol. Cell 14, 207. Hook, V., et al. 2004. Biol. Chem. 385, 473. Potts, W., et al. 2004. Int. J. Exp. Pathol. 85, 85. Rousselet, N., et al. 2004. Cancer Res. 64, 146. Schedel, J., et al. 2004. Gene Ther. 11, 1040. Troy, A.M. et al. 2004. Eur. J. Cancer 40, 1610. Corella, R., and Casey, S.F. 2003. Biotech. Histochem. 78, 101. Ohashi, K., et al. 2003. Biochim. Biophys. Acta 1649, 30. Friedrich, B., et al. 1999. Eur. J. Cancer 35, 138. Cresswell P., 1998. Science 280, 394. Yasuma, T., et al. 1998. J. Med. Chem. 41, 4301. Kirschke, H. et al. 1982. Biochem. J. 201, 367.

参考

Bohne, S., et al. 2004. J. Pathol.203, 528.
Goulet, B., et al. 2004. Mol. Cell 14, 207.
Hook, V., et al. 2004. Biol. Chem. 385, 473.
Potts, W., et al. 2004. Int. J. Exp. Pathol. 85, 85.
Rousselet, N., et al. 2004. Cancer Res. 64, 146.
Schedel, J., et al. 2004. Gene Ther. 11, 1040.
Troy, A.M. et al. 2004. Eur. J. Cancer 40, 1610.
Corella, R., and Casey, S.F. 2003. Biotech. Histochem. 78, 101.
Ohashi, K., et al. 2003. Biochim. Biophys. Acta 1649, 30.
Friedrich, B., et al. 1999. Eur. J. Cancer 35, 138.
Cresswell P., 1998. Science 280, 394.
Yasuma, T., et al. 1998. J. Med. Chem. 41, 4301.
Kirschke, H. et al. 1982. Biochem. J. 201, 367.

产品信息
检测方法	Fluorometric
形式	96 Tests
格式	96-well plate
试剂盒包含	Cathepsin L Substrate, AMC Calibration Standard, Cathepsin L Control, Cathepsin L Activator, CA 074, Cathepsin L Inhibitor, Assay Buffer, Sample Buffer, Diluent, Microwell Plate, Plate Sealer, and a user protocol.
正控制	Cathepsin L

生物信息
化验范围	1.56-100 ng/ml
化验时间	1.5 - 2 h
样品类型	Cell lysates, tissues, or purified cathepsin L

理化信息
灵敏度	1.2 ng/ml

产品使用声明
预定用途	The Calbiochem^® InnoZyme™ Cathepsin L Activity Kit, Fluorogenic is suited for measuring Cathepsin L activity using purified preparations, cell lysates, and tissue extracts, and for screening Cathepsin L inhibitors. This assay does not measure Cathepsin B activity.

储存和货运信息
货运代码	Dry Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
有害材料注意事项：	Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存	Multiple storage requirements
存储条件	Upon arrival store the Cathepsin L Control at -70°C; store all other unopened components at -20°C. Upon initial use of the kit, the components may be stored for up to 3 months under the following conditions: Store at 4°C: Assay Buffer, Sample Buffer Diluent, 96-Well Plate, and Plate Sealers Store at -20°C: AMC Calibration Standard, CA 074 Inhibitor, Cathepsin L Inhibitor Substrate, and Reduction Reagent Store at -70°C: Cathepsin L Control: dispense into aliquots upon initial use; avoid freeze/thaw cycles
避光	Protect from light
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No

补充信息
试剂盒包含	Cathepsin L Substrate, AMC Calibration Standard, Cathepsin L Control, Cathepsin L Activator, CA 074, Cathepsin L Inhibitor, Assay Buffer, Sample Buffer, Diluent, Microwell Plate, Plate Sealer, and a user protocol.