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merck millipore,默克密理博,FCMAB115F,Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate
产品名称：Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate
产品型号：FCMAB115F
Detect TRA-1-60 using this Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate validated for use in FC & IC.
merck millipore,默克密理博,FCMAB115F,Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, FC M FITC Monoclonal Antibody 描述 产品目录编号 FCMAB115F 描述 Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate 背景信息 Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein. 产品信息 格式 FITC 控制 - Pluripotent human embryonic stem (ES) cells
演示 Purified mouse monoclonal IgM conjugated to FITC in PBS with less than 0.09% sodium azide and 15 mg/mL BSA. 应用 应用 Detect TRA-1-60 using this Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate validated for use in FC & IC. 主要应用 - Immunocytochemistry
- Flow Cytometry
应用说明 Immunocytochemical staining of fixed H9 human ES cells incubated for two hours at 2-8ºC with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37ºC with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
Working Solution Cells / test Total Reaction Volume 5 μl 5 x 105 Cells / 95 μl PBS 100 μl 10 μl 1 x 106 Cells / 90 μl PBS 100 μl
生物信息 免疫原品种 - Whole Cells corresponding to the of TRA-1-60. 克隆 TRA-1-60 宿主 Mouse 特异性 This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
No immunoreactivity is seen with murine EC, EG, or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule同种型 IgM 品种反应性 Human Species Reactivity Note Tested and passed on Human. based on 100% sequence homology. 抗体类型 Monoclonal Antibody 基因符号 - TRA-1-60
纯化方法 Ammonium sulfate precipitation 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 tests
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