merck millipore,默克密理博,ES-002-D,EmbryoMax 2X Cell Culture Freezing Medium, Formulated with 20% DMSO & Fetal Bovine Serum

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Merck Millipore 默克密理愽

主要应用
Cult

描述
产品目录编号	ES-002-D
商名	Specialty Media Embryomax
描述	EmbryoMax® 2X Cell Culture Freezing Medium, Formulated with 20% DMSO & Fetal Bovine Serum
概述	This unique formulation of cell culture freezing media is for cryopreservation of a broad spectrum of mammalian cells (see viability data)and is ES cell qualified. Freezing Media contain 10% of the active cryopreservation ingredient (either DMSO or Glycerol). The remaining 90% is a proprietary mixture of Calf and Fetal Bovine Serum. The reagents used in these products are certified to be virus and mycoplasma free. The sera utilized in this medium is approved by the United States Department of Agriculture (USDA) to ensure that sera is free of diseases such as Bovine Spongiform Encephalopathy (BSE).

应用
应用	50ml EmbryoMax 2X Cell Culture Freezing Medium, Formulated with 20% DMSO & Fetal Bovine Serum optimized & validated for Stem Cell Culture.
主要应用	Stem Cell Culture
应用说明	Picking and freezing of ES cells: Wash Plate with PBS. Pick colonies into 96 wll plate (U shaped wells) with 35 µl of trypsin / well. Pick roughly 48 colonies, incubate at 37 °C for 10 minutes. Use Multichannel pipet to break up colonies. Transfer directly into 96 well plate with feeders and 150 µl of ES medium (without selection drug). Feed every day with ES medium containing selection drug (150 µg /ml neomycin, or 50 µg /ml of hygromycin). After 2 days cells should be ready to be frozen. Wash with PBS add 35 µl of Trypsin, incubate at 37 °C for 10 minutes, add 65 µl of ES medium. Use multichannel pipet to disperse cells. Transfer 65 µl into replica plate (U shaped wells) containing 65 µl of cold 2 x ES freezing medium. Wrap with parafilm, place on dry ice for 20 minutes, and transfer to -80 °C.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at -80°C up to 2 years

包装信息
数量	50 mL

merck millipore,默克密理博,ES-002-D,EmbryoMax 2X Cell Culture Freezing Medium, Formulated with 20% DMSO & Fetal Bovine Serum

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