- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
主要应用 Entrez基因编号 品种 Uni Prot编号 FUNC NM_002427.2 Human P45452 描述 产品目录编号 CC1047 品牌系列 Chemicon®
商名 - Chemicon
描述 MMP-13, human, proform, his-tagged, E. coli recombinant 概述 CC1047 is a recombinant 452 amino acid polypeptide corresponding to human Pro-MMP-13, with an additional C-terminal His-tag with the sequence GVTHHHHHH expressed in E. coli and purified from periplasm. The calculated Mr of the recombinant protein is 51.681 kDa. Upon activation with APMA activated MMP-13 is formed. Alternate Names - Procollagenase 3
背景信息 Matrix metalloproteinases (MMPs) are Zn2+- and Ca2+-dependent endopeptidases which function in the turnover of extracellular matrix components [Matrisian, 1992]. Main subfamilies of MMP are collagenases, gelatinases, stromelysins and membrane-type matrix metalloproteinases [Nagase, 1997]. Three homologous collagenases have been identified in human tissues: Interstitial collagenase, neutrophil collagenase and collagenase-3. These three enzymes cleave fibrillar collagens at a single site, generating fragments of approximately ¾ and ¼ the size of the original molecules.
ProMMP-13 [Procollagenase-3] consists of 452 amino acids with a calculated Mr of 52.520 [Freije et al., 1994]. Due to N-linked glycosylation, the actual Mr is about 60000 Da [Knauper et al., 1996]. Within the protein the following domains and sequence regions can be distinguished [Freije et al., 1994; Knauper et al., 1996]: An N-terminal propeptide, which confers latency to the proenzyme, a Ca2+ and Zn2+-ion binding catalytic domain, a hinge region, and a C-terminal hemopexin-like domain. Latent procollagenase-3 can be activated by proteases such as stromelysin [ Knauper et al., 1996], gelatinase A, MT1-MMP and plasmin [ Knauper et al., 1996] or incubation with APMA Knauper et al., 1996]. The Mr of active collagenase-3 which begins with the N-terminal sequence YNVFPRTL is 48,000 Da.
Collagenase-3 hydrolyzes type II collagen 5- to 6- times faster than type I and type III collagens. The enzyme also exhibits high activity towards gelatin and it degrades SERPINS as a1-antichymotrypsin and plasminogen activator inhibitor-2 [Knauper et al., 1996]. Collagenase-3 is inhibited in a 1:1 stoichiometric fashion by TIMP-1, TIMP-2 and TIMP-3.
Collagenase-3 is expressed during fetal bone development [Stahlebackdahl, 1997]. In adult human tissues collagenase-3 has been detected only in pathological conditions: in malignant tumors [Freije et al., 1994], in chronic ulcers [Valaamo et al., 1997], in arthritic cartilage [Mitchell et al., 1996] and synovium [Wernicke et al., 1996].
产品信息 演示 Provided as a liquid in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2, 0.05% Brij-35. 应用 主要应用 - Affects Function
应用说明 ACTIVATION:
An aliquot of 19.5 μL procollagenase-3 is mixed with 0.5 μL APMA solution (40 mM p-aminophenyl mercuric acetate in DMSO) and the mixture is incubated for 30 minutes at 37°C. The mixture may be stored on ice until use for activity assays.
INHIBITORS:
MMP-13 is inhibited by TIMPs and by chelators of divalent cations such as EDTA or o-phenanthroline.生物信息 浓缩 5 μg/25μL 纯度 Pro-MMP-13 appears as a major band at about 60 kDa in SDS-PAGE (>95% total protein). 品种 Human 特异活性 SPECIFIC ACTIVITY: <br /><br />The specific activity of activated MMP-13 is 250-300 mU/mg, where 1 U is the activity that hydrolyzes 1 μmol peptide (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Dpa-Ala-Arg) within 1 minute (Knight, 1992). Entrez基因编号 - NM_002427.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. 基因符号 - EC 3.4.24.-
- MMP13
- MMP-13
- CLG3
UniProt编号 - P45452
UniProt汇总 FUNCTION: SwissProt: P45452 # Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 471 amino acids; 53820 Da
TISSUE SPECIFICITY: Seems to be specific to breast carcinomas.
DOMAIN: SwissProt: P45452 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
DISEASE: SwissProt: P45452 # Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2 (SEMD2) [MIM:602111]; also known as spondyloepimetaphyseal dysplasia type Missouri. SEMDs are a heterogeneous group of skeletal disorders characterized by defective growth and modeling of the spine and long bones. The SEMDs are distinguished from the spondylometaphyseal dysplasias and the spondyloepiphyseal dysplasias by the combined involvement of the epiphyses and metaphyses. The 3 disorders have malformations of the vertebrae in common.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C in undiluted aliquots. The enzyme may be stored at -20°C for several weeks without significant loss of activity. Repeated freezing and thawing should be avoided. 包装信息 数量 5 µg
上一件merck millipore产品：merck millipore,默克密理博,07-1801,Anti-G3BP1 Antibody
下一件merck millipore产品：merck millipore,默克密理博,MAB7776,Anti-Urokinase Antibody, NT fragment, clone 2G6.2
相关产品
merck millipore,默克密理博,04-204,Anti-phospho-Cdc25C (Ser216) Antibody, clone E190, rabbit monoclonal
merck millipore,默克密理博,MAB8261-K,Anti-Influenza A, H1N1, clone 9B3.2
merck millipore,默克密理博,ABS381,Anti-GBP5 Antibody
merck millipore,默克密理博,MAB4310X,Anti-CD133 Antibody, clone 13A4, Alexa Fluor 488 conjugated
merck millipore,默克密理博,07-1801,Anti-G3BP1 Antibody
merck millipore,默克密理博,MAB7776,Anti-Urokinase Antibody, NT fragment, clone 2G6.2
merck millipore,默克密理博,681300,von Willebrand Factor, Human Plasma
merck millipore,默克密理博,ABE86,Anti-CHD-3 Antibody
