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检测方法 Colorimetric 描述 概述 Detects the dual phosphorylated forms of ERK1 at Thr202 and Tyr204 and ERK2 at Thr185 and Tyr187. This activation occurs as a result of treatment with a large variety of stimuli including mitogens, cytokines, and growth factors. Although this kit was designed for human samples, it cross-reacts with mouse and rat. 产品目录编号 CBA006 品牌系列 Calbiochem®
同义词 p44 MAP Kinase, Phospho-Specific (Thr¹⁸⁵/Tyr¹⁸⁷) ELISA 应用数据 
Jurkat cells were treated with 100 ng/ml PMA for 10 min and cell lysates were prepared. Cell lysate prepared with Cell Lysis Buffer was either boiled for 5 min, treated with Sample Treatment Buffer, or not treated. Cell lysate was also prepared using Denaturing Cell Lysis Buffer. Samples were analyzed with the ERK1/2 and the PhosphoDetect™ ERK (Thr185/Tyr187) ELISA Kits.
The sensitivity of this ELISA was compared to immunoblotting using known quantities of ERK1/2 Phospho-Thr185/Tyr187. The data shows that the sensitivity of the ELISA is approximately 4X greater than that of immunoblotting. The bands shown in the immunoblotting were developed using rabbit anti-ERK1/2 Phospho-Thr185/Tyr187 and an alkaline phosphatase conjugated anti-rabbit IgG followed by chemiluminescent detection.
Natural ERK1/2 Phospho-Thr185/Tyr187 from PMA-treated Jurkat cell lysates was serially diluted in Standard Diluent Buffer. The absorbance of each dilution was plotted against the ERK1/2 Phospho-Thr185/Tyr187 standard curve. Parallelism is demonstrated in the figure above and indicates that the standard accurately reflects natural ERK1/2 Phospho-Thr185/Tyr187 content in samples.参考 参考 Janulis, M., et al. 2001. Mol. Cell. Biol. 21, 2235.
Nanki, T., et al. 2001. J. Immunol. 167, 5381.
Sweatt, J.D. 2001. J. Neurochem. 76, 1.
Cross, T.G., et al. 2000. Exp. Cell Res. 256, 34.
Kolch, W. 2000. Biochem. J. 351, 289.
McCubrey, J.A., et al. 2000. Leukemia 14, 9.
Pearson, G., et al. 2000. J. Biol. Chem. 275, 37303.
Cobb, M.H., et al. 1994. Semin. Cancer Biol. 5, 261.
Cobb, M.H. and E.J. Goldsmith. 1995. J. Biol. Chem. 270, 14843.
Cobb, M.H. 1999. Prog. Biophys. Mol. Biol. 71, 479.
Impey, S., et al. 1999. Neuron 23, 11.
Payne, D.M., et al. 1991. EMBO J. 10, 885.
Tan, P.B., and S.K. Kim. 1999. Trends Genet. 15, 145.
Xu, S., et al. 1997. Mol. Endocrinol. 11, 1618.产品信息 检测方法 Colorimetric 形式 96 Tests 格式 96-well plate 试剂盒包含 Coated 96-Well Plate, ERK1/2 Phospho-Thr¹⁸⁵/Tyr¹⁸⁷ Standard, Diluents, Detector Antibody, Secondary Antibody, Sample Treatment Buffer, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol. 生物信息 化验范围 1.6-100 units/ml 化验时间 4 h 样品类型 Cell lysates 品种 human, mouse, rat 理化信息 灵敏度 ≤0.8 units/ml 安全信息 R相 R: 21/22-36/38
Harmful in contact with skin and if swallowed.
Irritating to eyes and skin.S相 S: 26-36/37-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).产品使用声明 预定用途 The Calbiochem® ERK1/2, Phospho-Specific (Thr¹⁸⁵/Tyr¹⁸⁷) ELISA is designed to detect and quantify the level of both dual-phosphorylated ERK2 at threonine 185 and tyrosine 187 (ERK2, Phospho-Specific Thr¹⁸⁵/Tyr¹⁸⁷) and ERK1 at threonine 202 and tyrosine 204 (ERK1, Phospho-Specific Thr²⁰²/Tyr²⁰⁴). The level of the phosphorylation of ERK1/2 can be an indirect indication of the activity of upstream kinases of ERK1/2 or the activity of ERK1/2 themselves. Although performance characterization of this ELISA kit is done primarily on human cell lines, this ELISA kit can be used for the detection of ERK1/2 in mouse and rat cells. This assay is intended for the detection of ERK1/2 dual phosphorylation from cell lysates. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 +2°C to +8°C
存储条件 Upon arricval store the entire contents of the kit at 4°C. 避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Coated 96-Well Plate, ERK1/2 Phospho-Thr¹⁸⁵/Tyr¹⁸⁷ Standard, Diluents, Detector Antibody, Secondary Antibody, Sample Treatment Buffer, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol.
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