merck millipore,默克密理博,APT110,Apo-Direct TUNEL Assay Kit

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merck millipore,默克密理博,APT110,Apo-Direct TUNEL Assay Kit
产品名称：Apo-Direct TUNEL Assay Kit
产品型号：APT110
The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.

merck millipore,默克密理博,APT110,Apo-Direct TUNEL Assay Kit

产品介绍

merck millipore,默克密理博,APT110,Apo-Direct TUNEL Assay Kit

重要规格表

主要应用	检测方法
FC	Fluorescent

描述
产品目录编号	APT110
品牌系列	Chemicon®
商名	Chemicon ApoDIRECT
描述	Apo-Direct TUNEL Assay Kit
概述	The CHEMICON APO-DIRECT™ Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.

产品信息
部件	PI/RNase Staining Buffer, Amber bottle The following components are included with the APO-DIRECT™ Kit. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions including control cells of approximately 1 x 106 cells per suspension for flow cytometry analysis. The control cells have been fixed as described (under the heading cell fixation procedure) and are in 70% (v/v) ethanol. Positive Control Cells,Brown cap Negative Control Cells, Natural cap Wash Buffer, Blue cap Reaction Buffer, Green cap TdT Enzyme, Yellow cap F-dUTP, Orange cap Rinsing Buffer, Red cap
检测方法	Fluorescent

产品信息

部件

PI/RNase Staining Buffer, Amber bottle
The following components are included with the APO-DIRECT™ Kit. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions including control cells of approximately 1 x 106 cells per suspension for flow cytometry analysis. The control cells have been fixed as described (under the heading cell fixation procedure) and are in 70% (v/v) ethanol.
Positive Control Cells,Brown cap
Negative Control Cells, Natural cap
Wash Buffer, Blue cap
Reaction Buffer, Green cap
TdT Enzyme, Yellow cap
F-dUTP, Orange cap
Rinsing Buffer, Red cap

检测方法

Fluorescent

应用
应用	The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.
主要应用	Flow Cytometry

生物信息
品种反应性	Vertebrates

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Precautions and Warnings: 1. The components of this kit are for Research Use only and are not intended for diagnostic procedures. 2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets. 3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.

储存和货运信息

存储条件

Precautions and Warnings:

1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.

2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.

3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.

包装信息
数量	50 assays

merck millipore,默克密理博,APT110,Apo-Direct TUNEL Assay Kit

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