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品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABT97 描述 Anti-KIF3B Antibody Alternate Names - Microtubule plus end-directed kinesin motor 3B
- Kinesin-like protein KIF3B
- HH0048
背景信息 Kinesin-like protein KIF3B (KIF3B) tethers chromosomes to the spindle pole and is involved in chromosomal movement. It forms a heterotrimeric protein with KIF3A and kinesin-like associated protein (KAP3). Both KIF3A and KIF3B contain an N-terminal globular motor domain, a potential neck coiled-coil region, a highly charged hinge region, a coiled-coil stalk region, and a C-terminal tail (The C-terminus region binds to KAP3). KIF3B interacts directly with certain proteins, such as CLC-5, IFT20, and the SMC3 subunit of the cohesion complex. It was shown to facilitate CLC-5 plasma membrane expression, endocytosis and microtubular transport in patients with Dent’s disease. 产品信息 格式 Affinity Purified 控制 - Human testis tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect the KIF3B protein using this Anti-KIF3B validated for use in WB & ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected KIF3B during telophase in A431 cells and during cytokinesis in HeLa cells. Punctate cytoplasmic staining has been observed in KIF3B-GFP transfected HEK293 cells (Reed, A. A., et al. (2010). Am J Physiol Renal Physiol. 298(2):F365-380.). 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human KIF3B. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. Predicted to react with Chicken, Bovine, Xenopus, Rat, Rhesus Macaque, and Mouse based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004789
基因符号 - KIF3B
纯化方法 Affinity Purified UniProt编号 - O15066
产品使用声明 质量保证 Evaluated by Western Blot in human testis tissue lysate.
Western Blot Analysis: 5 µg/mL of this antibody detected KIF3B in 10 µg of human testis tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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