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品种反应性 主要应用 宿主 格式 抗体类型 Chp, H, M, R ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABT63 描述 Anti-Ena/VASP-like protein Antibody Alternate Names - Ena/vasodilator-stimulated phosphoprotein-like
- Ena/VASP-like protein
背景信息 Ena (VASP-like protein) is involved in many different cellular functions including cell migration and axon guidance. Studies have shown that Ena expression is significantly increased in breast cancer and that the level of expression is associated with tumor stage. Ena has also been linked to inner ear and hair cell development. 产品信息 格式 Affinity Purified 控制 - Ramos cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect Ena/VASP-like protein using this Anti-Ena/VASP-like protein Antibody validated for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Ena/VASP-like protein in NIH/3T3 cells. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the EVH2 region of human Ena/VASP-like protein. 表位 EVH2 region 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Ena/VASP-like protein at the EVH2 region. 品种反应性 ChimpanzeeHumanMouseRat Species Reactivity Note Demonstrated to react with Human and Mouse.
Predicted to react with Rat and Chimpanzee based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_057421
基因符号 - RNB6
- EVL
纯化方法 Affinity Purified UniProt编号 - Q9UI08
UniProt汇总 FUNCTION: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization. Required to transform actin polymerization into active movement for the propulsive force of Listeria monocytogenes (By similarity).
SUBUNIT STRUCTURE: Homotetramer (By similarity). Binds to the SH3 domains of ABL1, LYN and SRC. Also binds to profilin, with preference for isoform IIa of PFN2, and the WW domain of APBB1/FE65. Binds to SEMA6A. Interacts, via the Pro-rich region, with the C-terminal SH3 domain of DNMBP. Interacts with RAPH1. Binds, via the EVH1 domain, the Pro-rich domain of Listeria monocytogenes actA (By similarity). Binds, via the EVH1 domain, the Pro-rich domain of ZYX.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton (By similarity). Cell projection › lamellipodium (By similarity). Note: Targeted to the leading edge of lamellipodia and the dital tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F-actin collar and the distal tip of microspikes (By similarity).
DOMAIN: The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
PTM: Phosphorylated by PKA; phosphorylation abolishes binding to SH3 domains of ABL and SRC (By similarity).
SEQUENCE SIMILARITIES: Belongs to the Ena/VASP family.
Contains 1 WH1 domain.产品使用声明 质量保证 Evaluated by Western Blot in Ramos cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Ena/VASP-like protein in 10 µg of Ramos cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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