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品种反应性 主要应用 宿主 格式 抗体类型 Chp, R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN27 Replaces 06-986 描述 Anti-GRIP-1 Antibody, CT Alternate Names - glutamate receptor-interacting protein 1
- glutamate receptor interacting protein 1
背景信息 Glutamate receptor interacting protein-1 (GRIP-1) is a multi-PDZ domain scaffold protein and a member of the steroid receptor coactivator (SRC) family of proteins. GRIP-1 interacts and maneuvers kinesin heavy chains to dendrites by acting as the power source for AMPA receptors. During initial stages of embryonic development, GRIP-1 is essential for typical cell-matrix interactions. 产品信息 格式 Affinity Purified 控制 - Rat brain cytosol tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect GRIP-1 using this Anti-GRIP-1 Antibody, C-terminus validated for use in WB, IP, IH(P). 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated GRIP-1 from rat brain cytosol tissue lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected GRIP-1 in rat brain cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the C-terminus of rat GRIP-1. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes GRIP-1 at the C-terminus. 品种反应性 ChimpanzeeRatHumanMouse Species Reactivity Note Demonstrated to react with rat. Predicted to react with human, mouse, and chimpanzee based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_114458
Entrez基因汇总 Binds to the liprin-alpha SVD2 family of proteins to mediate synaptic targeting of AMPA receptors; may play a role in presynaptic and postsynaptic maturation [RGD]. 基因符号 - CT
- GRIP
- GRIP-1
- GRIP1
纯化方法 Affinity purified UniProt编号 - P97879
UniProt汇总 FUNCTION: May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
SUBUNIT STRUCTURE: Interacts with EFNB1, EPHA7, EPHB2, EFNB3, KIF5A, KIF5C, KIF5B and the C-terminal tail of PRLHR. Forms a ternary complex with GRIA2 and CSPG4 (By similarity). Can form homomultimers or heteromultimers with GRIP2. Interacts with GRIA2, GRIA3, GRIPAP1/GRASP1, PPFIA1, PPFIA4, FRAS1, PLCD4, PTPRF and liprins-alpha. Interacts with SLC30A9 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Endomembrane system; Peripheral membrane protein. Cell junction › synapse › postsynaptic cell membrane. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Endoplasmic reticulum membrane; Peripheral membrane protein (Potential). Note: Membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
TISSUE SPECIFICTY: Expressed in brain, testis and retina. In brain highly expressed in the olfactory bulb, cortex and hyppocampus and lower level in thalamus, cerebellum and spinal cord. In brain it is found in the perikaryon, dendrites, dendritic shafts, dendritic spines and, excitatory and inhibitory synapses of neurons. In retina, it is most abundant in the plexiform layers than in perikarya.
DEVELOPMENTAL STAGE: Detected in early embryonic stage as early as E15, gradually increased throughout early development, peaked at approximately between postnatal days P6 and P8, then slightly decreased and remained relatively stable in the adult.
DOMAIN: PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with PRLHR (By similarity). PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1.
SEQUENCE SIMILARITIES: Contains 7 PDZ (DHR) domains.产品使用声明 质量保证 Evaluated by Western Blot in rat brain cytosol tissue lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected GRIP-1 on 10 µg of rat brain cytosol tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,102555,Abl Protein Tyrosine Kinase, Recombinant, E. coli
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