- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Po, H, M, R, Ca WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN101 描述 Anti-GAD65 Antibody Alternate Names - Glutamate decarboxylase 65 kDa isoform
- Glutamate decarboxylase 2
- 65 kDa glutamic acid decarboxylase
- GAD-65
背景信息 GAD65 is a group II decarboxylase enzyme that catalyzes the biosynthesis of GABA from glutamate with the aid of the cofactor, pyridoxal 5'-phosphate. GAD65 localizes at presynaptic GABAergic terminals and is reported to supply local vesicular pools of GABA facilitating transient inhibitiory neurotransmission, although GAD65 may also play a role in more sustained tonic inhibitory signals. It is present as a dimer of alpha and beta subunits and is regulated by alternative splicing, which may produce the GAD67 isoform. GAD65 also undergoes post-translational modifications such as palmitoylation of N-terminal cysteine residues and phosphorylation of multiple alpha-subunit serine residues that enable membrane-anchoring and other vesicular activities. GAD65 has been implicated in neurological conditions resulting from failure of inhibitory neurotransmission such as Huntington's disease and epilepsy. In addition to its CNS presence, GAD65 also produces GABA in pancreatic beta cells and dysfunction in this area may contribute to the pathogenesis of insulin-dependent diabetes mellitus. 产品信息 格式 Affinity Purified 控制 - Rat brain membrane tissue lysate
演示 Purified Rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-GAD65 Antibody is an antibody against GAD65 for use in Western Blotting. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: 1 µg/mL from a representative lot detected GAD65 on 10 µg of rat brain membrane tissue lysate. Western Blot performed without Tween. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human GAD65. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 PigHumanMouseRatCanine Species Reactivity Note Demonstrated to react with Rat, Mouse, and Human. Predicted to react with Canine and Porcine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000809
基因符号 - GAD2
- GAD65
纯化方法 Affinity purified UniProt编号 - Q05329
产品使用声明 质量保证 Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected GAD65 on 10 µg of rat brain membrane tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,375054,Heparin, Porcine Intestinal Mucosa, Biotin Conjugate - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,07-002,Anti-p67-phox Antibody
相关产品
merck millipore,默克密理博,CDUF006LT,Prep/Scale 卷式 TFF-6 膜包 PLTK 30 kDa 0.54 m2
merck millipore,默克密理博,SF-781,Beta Actin; Mouse, Cyanine 5, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,ABE446,Anti-Monomethyl Histone H1 (Lys26) Antibody
merck millipore,默克密理博,330200,Ecotin, E. coli - Calbiochem
merck millipore,默克密理博,375054,Heparin, Porcine Intestinal Mucosa, Biotin Conjugate - Calbiochem
merck millipore,默克密理博,07-002,Anti-p67-phox Antibody
merck millipore,默克密理博,PP54Z-K,Mouse IgG, Azide Free (1mg)
merck millipore,默克密理博,UFC900324,Amicon Ultra-15，PLBC Ultracel-PL 超滤膜，3 kDa
merck millipore,默克密理博,14-520M,Casein Kinase 1δ (aa 1-294) Protein, active, 250 µg
merck millipore,默克密理博,MAB2265,Anti-Peripheral Myelin Protein 22 (PMP22) Antibody, clone CF1