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  • merck millipore,默克密理博,ABE389,Anti-phospho-ATR (Ser428) Antibody

  • merck millipore,默克密理博,ABE389,Anti-phospho-ATR (Ser428) Antibody

    产品名称：Anti-phospho-ATR (Ser428) Antibody
    产品型号：ABE389
    Anti-phospho-ATR (Ser428) Antibody is a rabbit polyclonal antibody for detection of phospho-ATR (Ser428) also known as Serine/threonine-protein kinase ATR, Ataxia telangiectasia & Rad3-related protein More>>

    merck millipore,默克密理博,ABE389,Anti-phospho-ATR (Ser428) Antibody

  • 产品介绍
  • merck millipore,默克密理博,ABE389,Anti-phospho-ATR (Ser428) Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Mk, HWBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 ABE389
    描述 Anti-phospho-ATR (Ser428) Antibody
    Alternate Names
    • FRAP-related protein 1
    • Serine/threonine-protein kinase ATR
    • Ataxia telangiectasia and Rad3-related protein
    背景信息 The Ataxia-Telangiectasia and Rad3 related kinase (ATR) is a nuclear serine/threonine kinase that is part of the "first-response" system to DNA damage induced by a wide variety of factors including double-stranded breaks and replication stress. The localization of ATR to sites of DNA damage is aided by the Replication Protein A (RPA). Phospho - ATR (Ser428) targets a number of proteins including BRCA1, WRN, CHEK1, MCM2, and p53/TP53, and coordinates signaling pathways involved in DNA repair and apoptosis. ATR may also be involved in the phosphorylation of the histone H2A.X after replication stress, an early marker of DNA damage. However, ATR also mediates DNA replication and fork stability in normal cell cycles by activation of Chk1. Defective ATR contributes to Seckel syndrome type 1.
    产品信息
    格式 Affinity Purified
    控制
    • UV irradiated HeLa cell lysates and UV irradiated COS1 cell lysates
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Anti-phospho-ATR (Ser428) Antibody is a rabbit polyclonal antibody for detection of phospho-ATR (Ser428) also known as Serine/threonine-protein kinase ATR, Ataxia telangiectasia & Rad3-related protein, has been validated in WB.
    主要应用
    • Western Blotting
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to human ATR phosphorylated at (Ser428).
    表位 Phosphorylated (Ser428)
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody recognizes ATR phosphorylated at (Ser428).
    品种反应性 MonkeyHuman
    Species Reactivity Note Demonstrated to react in Human and Monkey. Other homologies: (63% mouse sequence homology).
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_001175
    Entrez基因汇总 The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.
    基因符号
    • FRP1
    • ATR
    纯化方法 Affinity purified
    UniProt编号
    • Q13535
    UniProt汇总 FUNCTION: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

    CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

    COFACTOR: Manganese.

    ENZYME REGULATION: Activated by DNA and inhibited by BCR-ABL oncogene. Slightly activated by ATRIP. Inhibited by caffeine, wortmannin and LY294002.

    SUBUNIT STRUCTURE: Forms an heterodimer with ATRIP. Binds to DNA, and to UV-damaged DNA with higher affinity. Interacts with RAD17, MSH2 and HDAC2. Present in a complex containing ATRIP and RPA-coated single-stranded DNA. Present in a complex containing CHD4 and HDAC2. Interacts with BCR-ABL after genotoxic stress. Interacts with EEF1E1. This interaction is enhanced by UV irradiation. Interacts with CLSPN and CEP164.

    SUBCELLULAR LOCATION: Nucleus. Note: Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.

    TISSUE SPECIFICITY: Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

    PTM: Phosphorylated; autophosphorylates in vitro.

    INVOLVEMENT IN DISEASE: Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.

    SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family. ATM subfamily.

    Contains 1 FAT domain.

    Contains 1 FATC domain.

    Contains 2 HEAT repeats.

    Contains 1 PI3K/PI4K domain.
    产品使用声明
    质量保证 Evaluated by Western Blot in UV irradiated HeLa cell lysates and UV irradiated COS1 cell lysates.

    Western Blot Analysis: A 1:200 dilution of this antibody detected ATR on 10 µg of UV irradiated HeLa cell lysates and UV irradiated COS1 cell lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 200 µL

    merck millipore,默克密理博,ABE389,Anti-phospho-ATR (Ser428) Antibody

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