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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB8221 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MMP-14 Antibody Alternate Names - MT1-MMP
背景信息 MMPs are a group of enzymes involved in matrix degradation. To date there are sixteen members reported to belong to the family of matrix metalloproteinases. Of these sixteen proteins ten exist in soluble form whereas MT-MMPs exist as integral membrane proteins. MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively contain a C-terminal transmembrane domain anchoring them to he cell surface. They have an 8 amino acid insert in their catalytic domain. MT1-MMP cleaves progelatinase A (MMP-2) and progelatinase B to their active forms. MT1-MMP gets activated through the membrane plasmin cascade. It binds to TIMP-2 first and then to MMP-2 forming a trimer. It cleaves the pro-MMP-2 to its active form. 产品信息 格式 Purified 控制 - POSITIVE CONTROL: HFL-1 Cells, Placenta
演示 Purified from rabbit anti-serum by Protein A chromatography. Prepared at 1mg/ml in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide 应用 应用 Anti-MMP-14 Antibody is an antibody against MMP-14 for use in WB. 主要应用 - Western Blotting
应用说明 Western Blotting: 5-10 μg/mL for 2 hrs at RT
The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator.生物信息 免疫原品种 A synthetic peptide corresponding to amino acids 319-333 of the human MMP-14/MT1-MMP 宿主 Rabbit 特异性 Recognizes a 60-66 kDa protein identified as MT1-MMP (MMP14).
CELLULAR LOCALIZATION: Cell Membrane and Cytoplasmic品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004995.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. 基因符号 - EC 3.4.24.80
- MMP14
- MTMMP1
- MMP-14
- MT1MMP
- MT1-MMP
- MMP-X1
UniProt编号 - P50281
UniProt汇总 FUNCTION: SwissProt: P50281 # Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity).
SIZE: 582 amino acids; 65884 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
DOMAIN: SwissProt: P50281 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 When stored at 2-8ºC, this antibody is stable for 12 months. 包装信息 数量 100 µg
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