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品种反应性 主要应用 宿主 格式 抗体类型 Pm, M, R IHC, ICC Gp Serum Polyclonal Antibody 描述 产品目录编号 AB5509 Replaces AB1774 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Opioid Receptor µ Antibody Alternate Names - MOR-1
产品信息 格式 Serum 演示 Guinea pig serum. Liquid containing 0.05% sodium azide. 应用 应用 Anti-Opioid Receptor μ Antibody-AB5509 is an antibody against Opioid Receptor for use in IC, IH. 主要应用 - Immunohistochemistry
- Immunocytochemistry
应用说明 Immunohistochemistry: 1:500 See Protocol.
Immunocytochemistry: 1:500 See Protocol.
Optimal working dilutions must be determined by the end user.
IHC Protocol: Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5509. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
ICC Protocol: Mu opioid receptor transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum free media. Media was removed and cells were gently washed 3 times with serum free media. Following fixation, cells were processed for indirect immunofluorescence as described above.生物信息 免疫原品种 Synthetic peptide (Catalog number AG375) from the C-terminus of rat mu Opioid receptor corresponding to residues 384-398 of the rat mu opioid receptor. 宿主 Guinea Pig 特异性 Mu Opioid receptor. 品种反应性 PrimateMouseRat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001008505.1
- NM_000914.2
- NM_001008503.1
- NM_001008504.1
基因符号 - KIAA0403
- OPRM1
- MOR-1
- MOR1
- OPRM
UniProt编号 - P35372
UniProt汇总 FUNCTION: SwissProt: P35372 # Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.
SIZE: 400 amino acids; 44779 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P35372 ## Belongs to the G-protein coupled receptor 1 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
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