merck millipore,默克密理愽
  • merck millipore,默克密理博,AB5490,Anti-Atrial Natriuretic Peptide Antibody

  • merck millipore,默克密理博,AB5490,Anti-Atrial Natriuretic Peptide Antibody

    产品名称：Anti-Atrial Natriuretic Peptide Antibody
    产品型号：AB5490
    This Anti-Atrial Natriuretic Peptide Antibody is validated for use in IH, IP, RIA for the detection of Atrial Natriuretic Peptide.

    merck millipore,默克密理博,AB5490,Anti-Atrial Natriuretic Peptide Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB5490,Anti-Atrial Natriuretic Peptide Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, MIHCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB5490
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Atrial Natriuretic Peptide Antibody
    Alternate Names
    • ANP
    产品信息
    格式 Affinity Purified
    控制
    • Heart tissue
    演示 Affinity Purified immunoglobulin. Liquid containing 0.1% sodium azide.
    应用
    应用 This Anti-Atrial Natriuretic Peptide Antibody is validated for use in IH, IP, RIA for the detection of Atrial Natriuretic Peptide.
    主要应用
    • Immunohistochemistry
    应用说明 Immunohistochemistry: 2-10 μg/mL. See suggested protocol below.

    Immunoprecipitation

    RIA

    Optimal working dilutions must be determined by the end user.

    IMMUNOHISTOCHEMISTRY PROTOCOL. Anesthetize animals and perfuse them transcardially as follows: a) Flush with cold (4°C) oxygenated Calcium free Tyrodes b) Perfuse with 4% formaldehyde in 0.16M phosphate buffer at pH 6.9 c) Perfuse with 10% sucrose solution in 0.1M phosphate buffer (pH 7.2)Sections Cut 5-30 mm sections using a cryostat and mount them on subbed histological slides.ImmunofluorescenceDilute ANP antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Triton X-100. Incubate sections for 24-48 hours at 2-8°C. Wash in PBS (three times for 10 minutes). Incubate for one hour or two hours at room temperature with properly diluted donkey anti-rabbit secondary antibody conjugated to a fluorescent probe such as FITC (Catalog Number AP182F), Rhodamine (Catalog Number AB182R) or Cy3 (Catalog Number AP182C). Wash in PBS (three times for 10 minutes) and mount with a PBS/glycerol solution containing 0.1% phenylenediamine to reduce fading. If not stained with FITC, sections can be dehydrated in grading alcohols (50%, 75%, 80%, 96% and 100%), cleared with xylene and mounted with DPX (Fluka, Ronkonkoma, NY). Staining can be visualized by using both conventional and confocal microscopy.Indirect Avidin-Biotin PeroxidaseIncubate sections with 0.3% H2O2 in PBS for 15 minutes at room temperature to block endogenous peroxidase. Rinse sections with PBS (three times for 10 minutes). Dilute ANP antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Triton X-100. Incubate sections overnight at 2-8°C and then wash in PBS (three times for 10 minutes). Incubate sections with properly diluted biotinilated goat anti-rabbit secondary antibody (Catalog Number AP132B) diluted in PBS (do not add sodium azide!) for one hour at room temperature. Rinse sections three times for 15 minutes and incubate sections with horseradish peroxidase-streptavidin complex (Catalog Number SA108) properly diluted in PBS/0.1% Triton X-100 for 40 minutes at room temperature. Wash sections PBS and incubate them in substrate solutions (e.g. DAB, AEC, etc.) to achieve necessary intensity of ANP staining.
    生物信息
    免疫原品种 Rat ANP conjugated to BSA.
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes Atrial Natriuretic Peptide (ANP).
    品种反应性 RatMouse
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_006172.2
    基因符号
    • Prepronatriodilatin
    • NPPA
    • CDD-ANF
    • ANP
    • PND
    • ANF
    • pronatriodilatin
    • OTTHUMP00000044485
    纯化方法 Immunoaffinity purified
    UniProt编号
    • P01160
    UniProt汇总 FUNCTION: SwissProt: P01160 # A potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis. Has a cGMP-stimulating activity.
    SIZE: 153 amino acids; 16708 Da
    SUBUNIT: Interacts with NPR3.
    SUBCELLULAR LOCATION: Secreted.
    SIMILARITY: SwissProt: P01160 ## Belongs to the natriuretic peptide family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,AB5490,Anti-Atrial Natriuretic Peptide Antibody

上一件merck millipore产品：merck millipore,默克密理博,121767,AG 1024 - CAS 65678-07-1 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,M04G-02-5PK,CellASIC ONIX gradient plate for mammalian cells (4 chamber)


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