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  • merck millipore,默克密理博,AB5472-200UL,Anti-Glycine Receptor α3 Subunit Antibody

  • merck millipore,默克密理博,AB5472-200UL,Anti-Glycine Receptor α3 Subunit Antibody

    产品名称：Anti-Glycine Receptor α3 Subunit Antibody
    产品型号：AB5472-200UL
    Detect Glycine Receptor α3 Subunit using this Anti-Glycine Receptor α3 Subunit Antibody validated for use in IH.

    merck millipore,默克密理博,AB5472-200UL,Anti-Glycine Receptor α3 Subunit Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB5472-200UL,Anti-Glycine Receptor α3 Subunit Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, MIHCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB5472-200UL
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Glycine Receptor α3 Subunit Antibody
    Alternate Names
    • GLRa3
    产品信息
    格式 Affinity Purified
    控制
    • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
    演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
    应用
    应用 Detect Glycine Receptor α3 Subunit using this Anti-Glycine Receptor α3 Subunit Antibody validated for use in IH.
    主要应用
    • Immunohistochemistry
    应用说明 Western blot: 1:200 on rat brain membranes.

    Immunohistochemistry on rat brain floating sections and mouse brain floating sections (trypsin pretreatment is recommended).

    Dilutions should be made using a carrier protein such as BSA (1-3%)

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 A purified peptide corresponding to amino acid residues 355-368 of rat GlyRa3 (Accession P24524).
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes Glycine receptor alpha3 (GlyRalpha3) protein. The epitope is not similar to any other known proteins.
    品种反应性 RatMouse
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_006529.2
    • NM_001042543.1
    基因符号
    • GLRA3
    UniProt编号
    • O75311
    UniProt汇总 FUNCTION: SwissProt: O75311 # The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
    SIZE: 464 amino acids; 53800 Da
    SUBUNIT: Pentamer composed of alpha and beta subunits (By similarity).
    SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
    TISSUE SPECIFICITY: Widely distributed throughout the central nervous system.
    DOMAIN: SwissProt: O75311 The N-terminal domain carries structural determinants essential for agonist and antagonist binding. TM2 is thought to form the innert wall of the chloride channel. The cytoplasmic loop is an important determinant of channel inactivation kinetics.
    SIMILARITY: Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
    MISCELLANEOUS: The alpha subunit binds strychnine.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 200 µL

    merck millipore,默克密理博,AB5472-200UL,Anti-Glycine Receptor α3 Subunit Antibody

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