merck millipore,默克密理愽
  • merck millipore,默克密理博,AB5150-50UL,Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT

  • merck millipore,默克密理博,AB5150-50UL,Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT

    产品名称：Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT
    产品型号：AB5150-50UL
    Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, C-terminus detects level of Calcium Channel & has been published & validated for use in IC, IP & WB.

    merck millipore,默克密理博,AB5150-50UL,Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT

  • 产品介绍
  • merck millipore,默克密理博,AB5150-50UL,Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB5150-50UL
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT
    产品信息
    格式 Affinity Purified
    演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
    应用
    应用 Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, C-terminus detects level of Calcium Channel & has been published & validated for use in IC, IP & WB.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    应用说明 In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each) (Westenbroek et al. 1992).

    Western blot: 1:200 using ECL on rat brain membranes.

    Immunohistochemistry using rat heart sections. Note: the epitope can be masked by other subunits in some Ca2+ channels.

    Dilutions should be made using a carrier protein such as BSA (1-3%)

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Purified peptide from {C-DNFDYLTRDWSILGPHHLD [CP15], cooresponding to residues 1506-1524 of rat alpha-1C {Accession P2202} subunit of skeletal muscle voltage-gated calcium channel (Striessnig et al. 1990), conjugated to KLH (Harlow & Lane 1988).
    表位 C-terminus
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes all types of alpha1 subunits of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. The epitope recongnized by the AB5150 is masked by alpha2 and delta chains, so this antibody can bind only after using Triton X 100 or other detergents that cause subunit dissociation (Ahlijanian et al. 1991).
    品种反应性 RatMouse
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_000719.5
    Entrez基因汇总 This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
    基因符号
    • CCHL1A1
    • CACNA1C
    • TS
    • MGC120730
    • CACH2
    • Cav1.2
    • CaV1.2
    • CACNL1A1
    • CACN2
    UniProt编号
    • Q13936
    UniProt汇总 FUNCTION: SwissProt: Q13936 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the high-voltage activated (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds.
    SIZE: 2221 amino acids; 248887 Da
    SUBUNIT: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore- forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with CACNA2D4.
    SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
    TISSUE SPECIFICITY: Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
    DOMAIN: SwissProt: Q13936 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. & Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel (By similarity).
    PTM: Phosphorylation by PKA activates the channel (By similarity).
    DISEASE: SwissProt: Q13936 # Defects in CACNA1C are the cause of Timothy syndrome (TS) [MIM:601005]. TS is a disorder characterized by multiorgan dysfunction including lethal arrhythmias, webbing of fingers and toes, congenital heart disease, immune deficiency, intermittent hypoglycemia, cognitive abnormalities and autism.
    SIMILARITY: Belongs to the calcium channel alpha-1 subunit family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB5150-50UL,Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT

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