merck millipore,默克密理博,AB175,Anti-GABA Antibody

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
A, R	ELISA, IH(P)	Gp	Serum	Polyclonal Antibody

描述
产品目录编号	AB175
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-GABA Antibody
背景信息	GABA (g-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system that is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.

产品信息
格式	Serum
控制	Rat Brain tissue
演示	Purified guinea pig polyclonal with 0.05% sodium azide.

应用
应用	This Anti-GABA Antibody is validated for use in Immunohistochemistry (Paraffin) and Enzyme Immunoassay (ELISA) for the detection of GABA.
主要应用	Enzyme Immunoassay (ELISA) Immunohistochemistry (Paraffin)
应用说明	Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABA in rat brain tissue. ELISA Analysis: A representative lot from an independent laboratory detected GABA in a competitive ELISA. Immunohistochemistry Protocol: Tissues fixed with 4% paraformaldehyde and 0-0.5% glutaraldehyde gives good results. Glutaraldehyde is required for antibody reactivity. 1) Tissue is fixed with 4% paraformaldehyde, 0-0.5% glutaraldehyde, 0.5% potassium dichromate in 0.1M phosphate buffer at pH 6.5. 2) Tissue is post-fixed overnight, vibratome sectioned in 50 mm and incubated in 0.05M Tris buffer, pH 6.5 for three hours. 3) Sections are incubated for 18-24 hours in AB175 diluted in PBS containing 0.1% sodium azide, 0.2% Triton X-100 and 1% normal goat serum. 4) Fluorescein conjugated antibody or ABC system may be used as the secondary reagent. Note: Without colchicine pretreatment well-stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.

生物信息
免疫原品种	BSA-conjugated GABA-Glutaraldehyde
浓缩	Variable
宿主	Guinea Pig
特异性	Recognizes GABA. Staining was blocked by preabsorbing with 100 μM GABA conjugated to glutaraldehyde. 500 μM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining.
品种反应性	AllRat
Species Reactivity Note	Demonstrated to react with Rat. GABA is a neurotransmitter synthesized in all species, and is therefore expected to react to all species.
抗体类型	Polyclonal Antibody
纯化方法	Depleted serum

产品使用声明
质量保证	Evaluated by Immunohistochemistry in rat brain tissue. Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected GABA in rat brain tissue.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

包装信息
数量	50 µL

merck millipore,默克密理博,AB175,Anti-GABA Antibody

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