merck millipore,默克密理博,AB1543P,Anti-Synapsin I Antibody

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merck millipore,默克密理博,AB1543P,Anti-Synapsin I Antibody
产品名称：Anti-Synapsin I Antibody
产品型号：AB1543P
This Anti-Synapsin I Antibody is validated for use in ELISA, IC, IH, IH(P), IP, WB for the detection of Synapsin I.

merck millipore,默克密理博,AB1543P,Anti-Synapsin I Antibody

产品介绍

merck millipore,默克密理博,AB1543P,Anti-Synapsin I Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, B, H, M	WB, ELISA, ICC, IHC	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	AB1543P
Replaces	MAB355
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Synapsin I Antibody
Alternate Names	Synapsin-1
背景信息	The synapsins are a family of proteins that have long been implicated in the regulation of neurotransmitter release at synapses. Specifically, they are thought to be involved in regulating the number of synaptic vesicles available for release via exocytosis at any one time. Synapsins are encoded by three different genes, synapsin I, II and III, and different neuron terminals will encode different amounts of these; synapsin will make up 1% of total brain protein at any one time. Current studies suggest the following hypothesis for the role of synapsin: synapsins bind synaptic vesicles to components of the cytoskeleton which prevents them from migrating to the presynaptic membrane and releasing transmitter. During an action potential, synapsins are phosphorylated by Ca2+/calmodulin-dependent protein kinase II, releasing the synaptic vesicles and allowing them to move to the membrane and release their neurotransmitter.

产品信息
格式	Purified
控制	Brain tissue.
演示	Purified rabbit polyclonal in buffer lyophilized from 5 mM ammonium bicarbonate. Reconstitute with 50 μL of PBS.

应用
应用	This Anti-Synapsin I Antibody is validated for use in ELISA, IC, IH, IH(P), IP, WB for the detection of Synapsin I.
主要应用	Western Blotting ELISA Immunocytochemistry Immunohistochemistry
应用说明	Western blotting: 1:200-1:1,000 dilution of a previous lot was used. Immunocytochemistry: 1:500-1:2,000 dilution of a previous lot was used. Immunoprecipitation: 1 μg of a previous lot immunoprecipitated all of the synapsin I from a SDS homogenate of 200 μg of rat brain protein. Note: The above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling. Immunohistochemistry: 1:500-1:2,500 ELISA: 1:2,500-1:10,00 Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Synapsin I (mixture of Ia & Ib) purified from bovine brain.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Synapsin I (Mr of 80,000 and 77,000). Synapsin is located only in brain nerve terminal and is an excellent marker for synapes. Immunolabeling is blocked by preadsorption of the antibody with synapsin I.
品种反应性	RatBovineHumanMouse
Species Reactivity Note	Human, bovine, rat, and mouse.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_006950.3
Entrez基因汇总	This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
基因符号	SYN1b SYN1 SYN1a SYNI
纯化方法	Immunoaffinity purified
UniProt编号	P17600
UniProt汇总	FUNCTION: SwissProt: P17600 # Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. SIZE: 705 amino acids; 74111 Da SUBUNIT: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 (By similarity). SUBCELLULAR LOCATION: Cell junction, synapse. PTM: Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: SwissProt: P17600 # Defects in SYN1 are a cause of X-linked recessive epilepsy associated with variable learning disabilities and behavior disorders [MIM:300491]. SIMILARITY: SwissProt: P17600 ## Belongs to the synapsin family.

产品使用声明
质量保证	Immunohistochemistry(paraffin): Synapsin I (AB1543P) representative staining pattern/morphology in rat hippocampal neurons. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. This lot of antibody was diluted to 1:1000, IHC-Select® reagents used with HRP-DAB. Immunoreactivity is seen directly associated with terminal end of neuronal cell body. IHC-Paraffin Staining With Epitope Retrieval: Rat Hippocampus
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Immunohistochemistry(paraffin):
Synapsin I (AB1543P) representative staining pattern/morphology in rat hippocampal neurons. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. This lot of antibody was diluted to 1:1000, IHC-Select® reagents used with HRP-DAB. Immunoreactivity is seen directly associated with terminal end of neuronal cell body.
IHC-Paraffin Staining With Epitope Retrieval:
Rat Hippocampus

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.