- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ca, R, M, Rhesus Macaque WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB10518 Replaces AB1656 描述 Anti-DARPP-32 Antibody Alternate Names - Dopamine- and cAMP-regulated neuronal phosphoprotein
- protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32)
- protein phosphatase 1, regulatory (inhibitor) subunit 1B
- dopamine and cAMP-regulated neuronal phosphoprotein 32
- dopamine and cAMP regulated phosphoprotein
- protein phosphatase 1 regulatory subunit 1B
背景信息 Dopamine- and cAMP-regulated neuronal phosphoprotein (DARP-32) is a cytoplasmic member of the protein phosphatase inhibitor 1 family. DARP-32 is a multifunctional protein kinase and protein phosphatase regulator, depending on the site of its activation. It possesses at least four sites that can be phosphorylated. Phosphorylation by PKA at Thr34 stimulates DARP-32 to act as a protein-phosphatase-1 (PP-1) inhibitor. However, phosphorylation at Thr75 will inhibit Thr34 phosphorylation. This selective, bidirectional regulation allows DARPP-32 to have a wide range of influence on downstream physiological effectors such as: ion pumps, neurotransmitter receptors, voltage-gate ion channels, and transcriptional factors. Studies suggest that it may also have a role in the Circadian System photic pathway. Overexpression of DARPP-32 has been linked with antiapoptotic mechanisms of cancer cells and has been observed in breast, colon, esophagus, gastric, and prostate cancer tissues. 产品信息 格式 Affinity Purified 控制 - Rat brain tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-DARPP-32 Antibody is an antibody against DARPP-32 for use in WB & IP. 主要应用 - Western Blotting
应用说明 Western Blot (Snap i.d.) Analysis: 1 µg/mL of this antibody from a previous lot detected DARPP-32 on 10 µg of rat brain tissue lysate.
Immunoprecipitaion Analysis: 10 µg of this antibody from a previous lot immunoprecipitated DARPP-32 from rat brain RIPA lysate.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to mouse DARPP-32 near the N-terminus. 表位 Near N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes DARPP-32 near the N-terminus. 品种反应性 CanineRatMouseRhesus Macaque Species Reactivity Note Demonstrated to react with rat and mouse. Predicted to react with rhesus monkey and canine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_659077
基因符号 - AG657
- DARPP32
- DARPP-32
- AB9208
- AB9206
纯化方法 Affinity purified UniProt编号 - Q60829
UniProt汇总 FUNCTION: Inhibitor of protein-phosphatase 1.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein (By similarity).
Phosphorylation of Thr-34 is required for activity (By similarity).
SEQUENCE SIMILARITIES: Belongs to the protein phosphatase inhibitor 1 family.产品使用声明 质量保证 Evaluated by Western Blot on rat brain tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected DARPP-32 on 10 µg of rat brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,71281,ProteoJuice™ Protein Transfection Reagent
下一件merck millipore产品：merck millipore,默克密理博,400110,Human IgA₂, Myeloma
相关产品
merck millipore,默克密理博,ECM535,α/β Integrin-mediated Cell Adhesion Array Combo Kit, fluorimetric
merck millipore,默克密理博,506166,p53 Modulator, CP-31398 - CAS 259199-65-0 - Calbiochem
merck millipore,默克密理博,06-1102,Anti-Protein patched homolog 1 Antibody
merck millipore,默克密理博,AB5772,Anti-Sox3 Antibody
merck millipore,默克密理博,71281,ProteoJuice™ Protein Transfection Reagent
merck millipore,默克密理博,400110,Human IgA₂, Myeloma
merck millipore,默克密理博,70-560,NFκB Family EZ-TFA Transcription Factor Assay Colorimetric Kit
merck millipore,默克密理博,S8005H,CpGenome™ 5-hmC DNA Standard
merck millipore,默克密理博,AB5991,Anti-Olig1 Antibody
merck millipore,默克密理博,12-510,Goat Anti-Rabbit IgG (H+L) Antibody, Rhodamine conjugate