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merck millipore,默克密理博,569372,PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6)
产品名称：PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6)
产品型号：569372
This PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of Src (pTyr⁴¹⁸).
merck millipore,默克密理博,569372,PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6)
- 产品介绍
重要规格表
宿主 M 描述 概述 Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 in chick embryo fibroblasts expressing human wild-type Src. 产品目录编号 569372 品牌系列 Calbiochem®
应用数据 
Detection of mouse Src, phosphorylated on Tyr423, by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells cultured under serum-free conditions, left untreated (lanes 1), or treated with H2O2 (lane 2). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (0.1 µg/ml) Detection: chemiluminescence.
Detection of human Src, phosphorylated on Tyr418, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (left lane) or treated with vanadate (right lane). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (0.1 µg/ml). Detection: chemiluminescence.
Detection of human Src, phosphorylated on Tyr418, by immunocytochemistry. Sample: A431 cells treated with EGF for 15 min. Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (1 µg/ml). Detection: fluorescence.参考 参考 Martin, G.S. 2001. Nat. Rev. Mol. Cell Biol. 2, 467.
Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol. 127, 226.
Bjorge, J.D., et al. 2000. Oncogene 19, 5620.
Irby, R.B. and Yeatman, T.J. 2001. Oncogene 19, 5636.
Miermont, A.M., et al. 2000. J. Immunol. Methods 246, 203.产品信息 形式 Lyophilized 配方 Lyophilized from 2X PBS, PEG, sucrose. 防腐剂 ≤0.1% sodium azide 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the pTyr⁴¹⁸ phosphorylation site of human Src 免疫原品种 Human 克隆 9A6 宿主 Mouse 同种型 IgG₁ 品种 human, mouse, rat 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Reconstitute in 1 ml sterile dH₂O (15 min, room temperature). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
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