merck millipore,默克密理博,16-232,Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate

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merck millipore,默克密理博,16-232,Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate
产品名称：Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate
产品型号：16-232
Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.

merck millipore,默克密理博,16-232,Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate

产品介绍

merck millipore,默克密理博,16-232,Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Av, H, R, Gp, B, M, Po	WB, FC, ICC	M	AlexaFluor®488	Monoclonal Antibody

描述
产品目录编号	16-232
品牌系列	Upstate
商名	Upstate
描述	Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 488 conjugate
Alternate Names	tubulin, alpha, brain-specific Alpha-tubulin 3 Tubulin B-alpha-1 Tubulin alpha-3 chain tubulin, alpha 1a tubulin, alpha 3 alpha 3 alpha
背景信息	Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.

产品信息
格式	AlexaFluor®488
控制	Included Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor® 488-conjugate.
演示	Alexa Fluor® 488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween®-20, 0.05% sodium azide. Liquid at 2-8°C.

应用
应用	Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.
主要应用	Western Blotting Flow Cytometry Immunocytochemistry
应用说明	Western Blot Analysis: 2 µg/mL of a previous lot detected α-Tubulin in RIPA lysates from A431 cells. Immunocytochemistry: 2 µg/mL of a previous lot showed positive immunostaining for α-Tubulin in HeLa cells.

生物信息
免疫原品种	Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
表位	The epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
克隆	DM1A
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes α-Tubulin, clone DM1A, Mr 50-60 kDa.
同种型	IgG1
品种反应性	AvianHumanRatGuinea PigBovineMousePig
Species Reactivity Note	Human, mouse, rat, bovine, porcine, guinea pig and avian.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_006000
Entrez基因汇总	Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
基因符号	hum-a-tub2 TUBA1A B-ALPHA-1 FLJ25113 hum-a-tub1 TUBA3
纯化方法	Protein G purfied
UniProt编号	Q71U36
UniProt汇总	FUNCTION:SwissProt: Q71U36 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE:451 amino acids; 50136 Da SUBUNIT: Dimer of alpha and beta chains. PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively (By similarity). SIMILARITY:SwissProt: Q71U36 ## Belongs to the tubulin family.

产品使用声明
质量保证	Routinely evaluated by Flow Cytometry. Flow Cytometry: 0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

产品使用声明

质量保证

Routinely evaluated by Flow Cytometry.

Flow Cytometry:
0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt. Do Not Freeze. Do not store the material diluted. For maximum recovery of product, centrifuge original vial prior to removing cap.