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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-881 描述 Anti-acetyl Cortactin Antibody Alternate Names - Src substrate cortactin
- Amplaxin
- Oncogene EMS1
背景信息 Cortactin is a cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 domain at the carboxyl terminus.
Recent research efforts show cortactin is acetylated in vivo and is deacetylated by HDAC6 (Histone deacetylase 6). HDAC6 is a tubulin-specific deacetylase that regulates microtubule-dependent cell movement. In recent studies it was demonstrated that HDAC6 binds cortactin and alters the ability of cortactin to bind F-actin by modulating a “charge patch” in its repeat region. Findings suggest that, in addition to its role in microtubule-dependent cell motility, HDAC6 influences actin-dependent cell motility by altering the acetylation status of cortactin, which, in turn, changes the F-actin binding activity of cortactin. The acetylation level of cortactin may correlate to tumor metastases and acetylated cortactin may be considered a potential metastasis marker.产品信息 格式 Affinity Purified 控制 - HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 This Anti-acetyl Cortactin Antibody is validated for use in WB, WB for the detection of acetyl Cortactin. 主要应用 - Western Blotting
生物信息 免疫原品种 Consensus sequence motif of acetylation sites of cortactin. 表位 Unknown 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody recognizes human acetylated Cortactin. 品种反应性 Human Species Reactivity Note Human and mouse. Predicted to react with rat and drosophila based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005222.2
Entrez基因汇总 This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq] 基因符号 - EMS1
- CTTN
修改 - Acetylation
纯化方法 Affinity Purfied UniProt编号 - Q14247
UniProt汇总 FUNCTION: May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
SUBUNIT STRUCTURE: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 By similarity. Interacts with PLXDC2.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton By similarity. Cell projection › lamellipodium By similarity. Cell projection › ruffle By similarity. Note: Associated with membrane ruffles and lamellipodia By similarity.
SEQUENCE SIMILARITIES: Contains 7 cortactin repeats.
Contains 1 SH3 domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide.
Western Blot Analysis: 2.5 µg/ml of this antibody detected Cortactin on 10 µL of HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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