merck millipore,默克密理博,08-143,SUMO2 Protein

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merck millipore,默克密理博,08-143,SUMO2 Protein
产品名称：SUMO2 Protein
产品型号：08-143

merck millipore,默克密理博,08-143,SUMO2 Protein

产品介绍

merck millipore,默克密理博,08-143,SUMO2 Protein

描述
产品目录编号	08-143
品牌系列	Upstate
商名	Upstate
描述	SUMO2 Protein

产品信息
演示	100 µg of concentration 1.0 mg/mL in 20mM HEPES, pH8.0, 50mM NaCl, 1mM DTT.

应用
应用说明	Mature SUMO-2 suitable for in vitro SUMOylation using the SUMO Activating Enzyme Complex (Cat. no. 14-858) and SUMO conjugating enzyme UbcH9 (Cat. no. 14-815). In The presence of these enzymes with ATP, Mg2+, and DTT, mature SUMO-2 may be conjugated to soluble proteins. The protein is N-terminally His6-tagged within a leader sequence. Typical in vitro concentration for non-rate limiting conjugate formation is 200μM to 1mM depending upon conditions.

应用

应用说明

Mature SUMO-2 suitable for in vitro SUMOylation using the SUMO Activating Enzyme Complex (Cat. no. 14-858) and SUMO conjugating enzyme UbcH9 (Cat. no. 14-815). In The presence of these enzymes with ATP, Mg2+, and DTT, mature SUMO-2 may be conjugated to soluble proteins. The protein is N-terminally His6-tagged within a leader sequence. Typical in vitro concentration for non-rate limiting conjugate formation is 200μM to 1mM depending upon conditions.

生物信息
浓缩	1.0 mg/mL
纯度	≥95%
品种	Human
Entrez基因编号	NM_006936.2
Entrez基因汇总	SUMO proteins, such as SUMO3, and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (Su and Li, 2002 [PubMed 12383504]).[supplied by OMIM]
基因符号	SUMO-3 SUMO3 SUMO-2 SMT3H1 SMT3A
UniProt编号	P55854
UniProt汇总	FUNCTION: SwissProt: P55854 # Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. SIZE: 103 amino acids; 11637 Da SUBUNIT: Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins (By similarity). SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed predominantly in liver. PTM: Polymeric chains can be formed through Lys-11 cross-linking. & Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function. SIMILARITY: SwissProt: P55854 ## Belongs to the ubiquitin family. SUMO subfamily. & Contains 1 ubiquitin-like domain.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -80°C from date of shipment for up to 12 months. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80ºC.