merck millipore,默克密理博,07-729,Anti-CTCF Antibody

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merck millipore,默克密理博,07-729,Anti-CTCF Antibody
产品名称：Anti-CTCF Antibody
产品型号：07-729
Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor.

merck millipore,默克密理博,07-729,Anti-CTCF Antibody

产品介绍

merck millipore,默克密理博,07-729,Anti-CTCF Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, Ca, M, Pm, H	ChIP, WB	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	07-729
Replaces	MABE547
品牌系列	Upstate
商名	Upstate
描述	Anti-CTCF Antibody
Alternate Names	transcriptional repressor CTCF 11 zinc finger transcriptional repressor 11-zinc finger protein CCCTC-binding factor CCCTC-binding factor (zinc finger protein) CTCFL paralog
背景信息	CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma.

产品信息
格式	Serum
控制	HeLa nuclear extract. Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.
演示	Rabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol.

应用
应用	Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation: An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells.

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes.
浓缩	The concentration of this serum antibody has not been determined.
宿主	Rabbit
特异性	Recognizes CTCF at a.a. 659-675.
品种反应性	RatCanineMousePrimateHuman
Species Reactivity Note	Human. Predicted to cross-react with mouse, rat, dog and primates based on sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_006565.2
Entrez基因汇总	This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.
基因符号	CTCF
纯化方法	Unpurified
UniProt编号	P49711
UniProt汇总	FUNCTION:SwissProt: P49711 # Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation- dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor. SIZE: 727 amino acids; 82785 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY:Ubiquitous. Absent in primary spermatocytes. SIMILARITY: SwissProt: P49711 ## Belongs to the CTCF zinc-finger protein family. & Contains 11 C2H2-type zinc fingers.

产品使用声明
质量保证	Routinely evaluated by Western Blot. Western Blot Analysis: A 1:1000–1:5000 dilution of this lot detected CTCF in HeLa nuclear extract. A previous lot detected CTCF in K562 nuclear extract (data not shown).
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.