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品种反应性 主要应用 宿主 格式 抗体类型 Gp, M, Rhesus Macaque ChIP Rb Serum Polyclonal Antibody 描述 产品目录编号 07-1802 描述 Anti-TAF3 Antibody Alternate Names - TAFII140
- Transcription initiation factor TFIID subunit 3
- 140 kDa TATA box-binding protein-associated factor
- TBP-associated factor 3<br />Transcription initiation factor TFIID 140 kDa subunit
- TAF(II)140
- TAF140
背景信息 TAF3 is a subunit of TFIID that was initially identified through its interaction with a developmentally regulated transcription factor Bric a brac 1 (bab1), which is implicated in pattern formation and tissue morphogenesis in Drosophila. TFIID is a general or basal transcription factor that regulates initiation by allowing RNA polymerase II to recognize a core promotor. TAF3-TBPL2 complexes are found in myoblasts; however, upon differentiation into myotubes, they are replaced by TRF3-TAF3 complexes. Further research is still required to elucidate the roles and the development of the former to the latter complex. 产品信息 格式 Serum 控制 - C2C12 cell lysate.
演示 Rabbit polyclonal serum containing with 0.05% sodium azide. 应用 应用 Detect TAF3 using this Anti-TAF3 Antibody validated for use in 0. 主要应用 - Chromatin Immunoprecipitation (ChIP)
应用说明 Chromatin Immunoprecipitation: A representative lot immunoprecipitated TAF3 using chromatin from transfected cells grown under conditions that favor expression from the MyoD:EGFP transgene promoter over expression from the endogenous MyoD promoter. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human TAF3. 宿主 Rabbit 品种反应性 Guinea PigMouseRhesus Macaque Species Reactivity Note Demonstrated to react with Mouse. Predicted to react with Human, Rhesus Macaque and Guinea Pig based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_114129.1
Entrez基因汇总 The highly conserved RNA polymerase II transcription factor TFIID (see TAF1; MIM 313650) comprises the TATA box-binding protein (TBP; MIM 600075) and a set of TBP-associated factors (TAFs), including TAF3. TAFs contribute to promoter recognition and selectivity and act as antiapoptotic factors. 基因符号 - TAF3
纯化方法 Unpurified UniProt编号 - Q5VWG9
UniProt汇总 FUNCTION: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.
SUBUNIT STRUCTURE: Belongs to the TFIID complex which is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TAF10 via the histone fold. Interacts with TAF13, TBP, SAP130 and GCN5L2. Interacts with TBPL2.
SUBCELLULAR LOCATION: Nucleus.产品使用声明 质量保证 Evaluated by Immunoprecipitation in C2C12 cell lysate.
Immunoprecipitation Analysis: A 1:1,000 dilution of this antibody detected TAF3 in 500 µg of C2C12 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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