merck millipore,默克密理博,07-1719,Anti-FOXO3A Antibody

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merck millipore,默克密理博,07-1719,Anti-FOXO3A Antibody
产品名称：Anti-FOXO3A Antibody
产品型号：07-1719
This Anti-FOXO3A Antibody is validated for use in WB, IC for the detection of FOXO3A.

merck millipore,默克密理博,07-1719,Anti-FOXO3A Antibody

产品介绍

merck millipore,默克密理博,07-1719,Anti-FOXO3A Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	ICC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-1719
Replaces	04-1007
描述	Anti-FOXO3A Antibody
Alternate Names	forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 AF6q21 protein Forkhead in rhabdomyosarcoma-like 1 forkhead box O3 forkhead box O3A forkhead homolog (rhabdomyosarcoma) like 1 forkhead Drosophila homolog of
背景信息	Forkhead box O3A (FOXO3A) is a member of the forkhead family of transcription factors (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. Upon oxidative stress, FOXO3A translocates to the nucleus where binding to the DNA sequence 5’-[AG]TAAA[TC]A-3’ occurs and IGF-1 is dephosphlorated, resulting in apoptosis. In order for the cell to survive, IGF-1 must be phosphlorated by AKT1/PKB at Thr32 and Ser-253, which will result in retention and inactivation of FOXO3A in the cytoplasm. Translocation of FOXO3A with the MLL gene has been associated with secondary acute leukemia.

产品信息
格式	Affinity Purified
控制	FOXO3A in Jurkat cell lysate.
演示	Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

应用
应用	This Anti-FOXO3A Antibody is validated for use in WB, IC for the detection of FOXO3A.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry: A 1:500 dilution of this antibody was used to detect FOXO3A in HeLa cells. Positive staining in the nucleus.

生物信息
免疫原品种	Synthetic peptide corresponding to FOX03A
宿主	Rabbit
特异性	This antibody recognizes FOXO3A.
品种反应性	MouseHuman
Species Reactivity Note	Tested on human. Predicted to react with mouse based on sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001446
Entrez基因汇总	This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq].
基因符号	OTTHUMP00000016944 AF6q21 DKFZp781A0677 FKHRL1 FKHRL1P2 FOXO2 FOXO3A MGC12739 MGC31925
纯化方法	Affinity Purfied
UniProt编号	O43524
UniProt汇总	FUNCTION:Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Ref.5 Ref.7 SUBUNIT: Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. Ref.7 SUBCELLULAR LOCATION:Cytoplasm › cytosol. Nucleus. Note= Translocates to the nucleus upon oxidative stress and in the absence of survival factors. Ref.5 Ref.7 TISSUE SPECIFICITY: Ubiquitous. Ref.1 PTM: In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Ref.5 Ref.7 Ref.6 Ref.8 Ref.9 DISEASE: A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. SIMILARITY: Contains 1 fork-head DNA-binding domain.

产品使用声明
质量保证	Western Blot Analysis: 1:500 dilution of this antibody was used to detect FOXO3A in Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8ºC from date of receipt. To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

储存和货运信息

存储条件

Stable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.