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merck millipore,默克密理博,07-1568,Anti-ESET/SetDB1 Antibody
产品名称：Anti-ESET/SetDB1 Antibody
产品型号：07-1568
Use Anti-ESET/SetDB1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect ESET/SetDB1 also known as SET domain bifurcated 1, ERG-associated protein with SET domain.
merck millipore,默克密理博,07-1568,Anti-ESET/SetDB1 Antibody
- 产品介绍
Replacement Information Replacement Information This product is a recommended replacement for 07-378. 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1568 Replaces 07-378 品牌系列 Upstate
商名 - Upstate
描述 Anti-ESET/SetDB1 Antibody Alternate Names - EC 2.1.1.43<br />SET domain bifurcated 1<br />ERG-associated protein with SET domain<br />ESET<br />Histone H3-K9 methyltransferase 4<br />H3-K9-HMTase 4<br />Lysine N-methyltransferase 1E
- SETDB1
背景信息 The SET domain, bifurcated 1, also known as SETDB1, is a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. mAM facilitates conversion by SETDB1 of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression. Additionally, SETDB1 and the DNA methyltransferase DNMT3A interact directly and localize to promoters silenced in cancer cells. 产品信息 格式 Purified 应用 应用 Use Anti-ESET/SetDB1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect ESET/SetDB1 also known as SET domain bifurcated 1, ERG-associated protein with SET domain. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic peptide (AGSRTPKDQKLREA(C) corresponding to a.a. 140-153 of human SETDB1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes ESET/SetDB1, Mr 180 kDa. 品种反应性 Human Species Reactivity Note Expected to recognize mouse, horse, pig, and dog, based on sequence identity. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_012432
Entrez基因汇总 The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.[supplied by OMIM] UniProt编号 - Q15047
UniProt汇总 FUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine.
SUBUNIT: Interacts with MBD1; interaction is abolished when MBD1 is sumoylated. Interacts with ATF7IP and ATF7IP2; the interaction with ATF7IP is required to stimulate histone methyltransferase activity and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9'. During DNA replication, it is recruited by SETDB1 to form a S phase-specific complex that facilitates methylation of H3 'Lys-9' during replication-coupled chromatin assembly and is at least composed of the CAF-1 subunit CHAF1A, MBD1 and SETDB1. Interacts with ERG, TRIM28/TIF1B, CBX1, CBX5, DNMT3A, HDAC1, HDAC2, SIN3A, SIN3B, DNMT3B and SUMO2.
SUBCELLULAR LOCATION: Nucleus. Note=Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
Excluded from nucleoli and islands of condensed chromatin
Miscellaneous: Isoform 2 lacks all domains required for histone methyltransferase activity
Miscellaneous: Highly up-regulated in Huntington disease patients, suggesting that participates in the altered chromatin modulation and transcription dysfunction observed in Huntington disease. Its down-regulation has salubrious effects on patients, suggesting that it may be a promising treatment in Huntington disease patients
产品使用声明 质量保证 Routinely evaluated by immunoblot on HeLa nuclear extract 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 100 µg
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