merck millipore,默克密理博,07-1420,Anti-VEGF Antibody

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merck millipore,默克密理博,07-1420,Anti-VEGF Antibody
产品名称：Anti-VEGF Antibody
产品型号：07-1420
Anti-VEGF Antibody detects level of VEGF & has been published & validated for use in WB, IH(P), ELISA.

merck millipore,默克密理博,07-1420,Anti-VEGF Antibody

产品介绍

merck millipore,默克密理博,07-1420,Anti-VEGF Antibody

Replacement Information
Replacement Information	07-1420 is a recommended replacement for AB2142P.

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	ELISA, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-1420
Replaces	AB2142P
描述	Anti-VEGF Antibody
Alternate Names	Vascular permeability factor
背景信息	VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.

产品信息
格式	Affinity Purified
控制	Huvec cell lysate
演示	Affinity purified rabbit polyclonal IgG employing immobilized mouse VEGF matrix. Antibody solution (1 mg/ml) was lyophilized from PBS, pH 7.2. Reconstitute with sterile distilled water.

应用
应用	Anti-VEGF Antibody detects level of VEGF & has been published & validated for use in WB, IH(P), ELISA.
主要应用	ELISA Western Blotting
应用说明	Immunohistochemisty (paraffin): VEGF staining in mouse placenta, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of antibody was diluted to 1:100, using IHC-Select® detection with HRP-DAB. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mouse VEGF (50.0 ng/mL), a concentration of 1.0-2.0 µg/mL of a previous lot of this antibody was required. ELISA: A concentration of at least 0.5 µg/mL (100 μL/well) is required to detect 0.2-0.4 ng/well of recombinant mouse VEGF using a previous lot of this antibody. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Highly pure (>98%) recombinant mouse Vascular Endothelial Growth Factor.
宿主	Rabbit
特异性	Recognizes Vascular Endothelial Growth Factor (VEGF), a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Human and Mouse
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_001025366.1
Entrez基因汇总	This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG,leading to additional isoforms. [provided by RefSeq].
基因符号	VEGF VEGFA VPF VEGF-A
纯化方法	Affinity Purfied
UniProt编号	P15692
UniProt汇总	FUNCTION: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity). SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. ALTERNATIVE PRODUCTS: 10 named isoforms [FASTA] produced by alternative splicing.

产品使用声明
质量保证	Routinely evaluated by Western Blot on Huvec lysates. Western Blot Analysis: 1:500 dilution of this lot detected VEGF on 10 μg of Huvec lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Reconstituted antibody, at a concentration of ≥ 0.2 mg/mL, is stable for at least six weeks at 2-8ºC. Aliquots stored at -20ºC are stable for at least one year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

储存和货运信息

存储条件

Reconstituted antibody, at a concentration of ≥ 0.2 mg/mL, is stable for at least six weeks at 2-8ºC. Aliquots stored at -20ºC are stable for at least one year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.