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merck millipore,默克密理博,06-863,Anti-CREB Antibody
产品名称：Anti-CREB Antibody
产品型号：06-863
Anti-CREB Antibody is a Rabbit Polyclonal Antibody for detection of CREB also known as active transcription factor CREB, cAMP-response element-binding protein-1, transactivator protein & has been vali More>>
merck millipore,默克密理博,06-863,Anti-CREB Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 06-863 Replaces 04-218 品牌系列 Upstate
商名 - Upstate
描述 Anti-CREB Antibody Alternate Names - transactivator protein
- active transcription factor CREB
- cAMP responsive element binding protein 1
- cAMP-response element-binding protein-1
背景信息 CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of Neurotrophin pathways. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2. 产品信息 格式 Purified 控制 - HeLa nuclear extract
Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, and 30% glycerol. 应用 应用 Anti-CREB Antibody is a Rabbit Polyclonal Antibody for detection of CREB also known as active transcription factor CREB, cAMP-response element-binding protein-1, transactivator protein & has been validated in IP & WB. 主要应用 - Western Blotting
应用说明 Immunoprecipitation:
5 μg of this lot immunoprecipitated CREB from 500 μg of HeLa nuclear extract.生物信息 免疫原品种 KLH-conjugated, synthetic peptide (CSGAENQ QSGDAAVTEAENQQ) corresponding to a.a. 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat. 表位 a.a. 5-24 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes CREB. 同种型 IgG 品种反应性 MouseHuman Species Reactivity Note Human and mouse.
Broad species cross-reactivity expected due to conservation of immunogenic sequence.抗体类型 Polyclonal Antibody Entrez基因编号 - NM_134442
Entrez基因汇总 This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. 基因符号 - CREB1
- MGC9284
纯化方法 Protein A purfied UniProt编号 - P16220
UniProt汇总 FUNCTION: SwissProt: P16220 # This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE.
SIZE: 341 amino acids; 36688 Da
SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.
SUBCELLULAR LOCATION: Nucleus.
PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.产品使用声明 质量保证 Routinely evaluated by western blot on HeLa nuclear lysate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected CREB from a HeLa nuclear lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing cap.包装信息 数量 200 µg
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