merck millipore,默克密理博,06-598,Anti-acetyl-Histone H4 Antibody

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merck millipore,默克密理博,06-598,Anti-acetyl-Histone H4 Antibody
产品名称：Anti-acetyl-Histone H4 Antibody
产品型号：06-598
Anti-acetyl-Histone H4 Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a & has been validated in ChIP, ICC, IP & WB.

merck millipore,默克密理博,06-598,Anti-acetyl-Histone H4 Antibody

产品介绍

merck millipore,默克密理博,06-598,Anti-acetyl-Histone H4 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H, T	ChIP, ICC, WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	06-598
品牌系列	Upstate
商名	Upstate
描述	Anti-acetyl-Histone H4 Antibody
Alternate Names	histone cluster 1, H4a H4 histone family, member A histone 1, H4a histone 1 H4a
背景信息	Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

产品信息
格式	Purified
控制	TSA-treated NIH/3T3, A431, L929 (mouse) and C6 (rat) cell extracts, human breast carcinoma.
演示	Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.

应用
应用	Anti-acetyl-Histone H4 Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a & has been validated in ChIP, ICC, IP & WB.
主要应用	Chromatin Immunoprecipitation (ChIP) Immunocytochemistry Western Blotting
应用说明	Immunoprecipitation: 5 μg of a previous lot immunoprecipitated acetylated histone H4 from 500 μg of acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours. Immunocytochemistry: 5-10 μg/mL of a previous lot lot gave positive nuclear immunostaining in human A431 cells treated with sodium butyrate and fixed with ice-cold 1% paraformaldehyde in PBS. Chromatin Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate acetylated histone H4 from chromatin.

生物信息
免疫原品种	KLH-conjugated peptide corresponding to amino acids 2-19 of Tetrahymena histone H4 (AGG(Ac)KGG(Ac)KGMG(Ac)KVGA(Ac)KRHS-C), acetylated on lysines 5,8,12 and 16.
表位	a.a. 2-19
浓缩	1 mg/mL
宿主	Rabbit
特异性	Recognizes acetylated histone H4. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May crossreact with other acetylated proteins.
同种型	IgG
品种反应性	HumanTetrahymena
Species Reactivity Note	Human and Tetrahymena. Other species not tested.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_175054
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq]
基因符号	H4/E HIST1H4A H4/J HIST1H4F HIST1H4L H4FN H4FA H4FH HIST1H4H H4F2 H4FM H4/A H4FK H4FG HIST2H4 H4FB H4/K HIST1H4I H4/N HIST1H4B H4FE H4/M H4/a HIST1H4E HIST4H4 HIST2H4A H4FC H4FI H4/H HIST1H4C H4/G HIST1H4K H4FJ H4FD H4/I H4/B H4/D H4/C HIST1H4J HIST1H4D
修改	Acetylation
纯化方法	Protein A chromatography
UniProt编号	P62805
UniProt汇总	FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 103 amino acids; 11367 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.

产品使用声明
质量保证	Routinely evaluated by western blot on acid-extracted proteins from HeLa cells treated with sodium butyrate. Western Blot Analysis: 0.5-2 μg/mL of this lot detected acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate. A previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Routinely evaluated by western blot on acid-extracted proteins from HeLa cells treated with sodium butyrate.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate. A previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.