merck millipore,默克密理博,05-947,Anti-Phosphotyrosine Antibody, clone PY20

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Merck Millipore 默克密理愽

Replacement Information
Replacement Information	05-947 is a recommended replacement for MAB3080

品种反应性	主要应用	宿主	格式	抗体类型
Vrt	WB, ELISA	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-947
Replaces	MAB3080
品牌系列	Upstate
商名	Upstate
描述	Anti-Phosphotyrosine Antibody, clone PY20
背景信息	Tyrosine phosphorylation represents a major mechanism for the activation of signal transduction pathways in response to growth factors, hormones and cytokines. There are approximately 100 tyrosine kinases that are both receptor tyrsosine kinase receptors for various growth factors and cytokines. The extent and duration of tyrosine phosphorylation are tightly regulated by the 107 protein tyrosine phosphatases. Tyrosine phosphorylation occurs on residues on either other kinases (Ser/Thr or Tyr) or on substrates. In the case of kinases, this phosphorylation often causes the enzyme to change activation states and/or it creates binding sites for SH2 domain. Mutation or misregulation of protein tyrosine kinases is a cause or consequence of many disease states. It has been estimated that 20% of oncogenes so far described are tyrosine kinases. With such clear links between aberrant protein tyrosine kinase activity and disease, it is no surprise that much of the early effort in kinase drug discovery was focused on these enzymes.

产品信息
格式	Purified
控制	EGF-stimulated A431 cell lysate. Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
演示	0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4. Liquid at 2-8°C.

应用
应用	Anti-Phosphotyrosine Antibody, clone PY20 is an antibody against Phosphotyrosine for use in ELISA, IP & WB.
主要应用	Western Blotting ELISA
应用说明	Immunoprecipitation: Recommended by an independent laboratory to immunoprecipitate tyrosine phosphorylated proteins. ELISA: Recommended by an independent laboratory.

生物信息
免疫原品种	Phosphotyrosine conjugated to carrier protein.
克隆	PY20
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Tyrosine-phosphorylated proteins from all species.
同种型	IgG2b
品种反应性	Vertebrates
Species Reactivity Note	Most common vertebrate species tested. Reactivity with other species has not been confirmed.
抗体类型	Monoclonal Antibody
修改	Phosphorylation
纯化方法	Protein A purfied

产品使用声明
质量保证	Routinely evaluated by western blot with RIPA lysates EGF-treated human A431 carcinoma cells. Western Blot Analysis: 0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in RIPA lysates EGFtreated human A431 carcinoma cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of recipt. NOTE: DO NOT FREEZE. For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.

包装信息
数量	1 mg

merck millipore,默克密理博,05-947,Anti-Phosphotyrosine Antibody, clone PY20

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