merck millipore,默克密理博,05-1574,Anti-Rab25 Antibody, clone 12C3

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
Rb, H, M, R	IHC, WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-1574
描述	Anti-Rab25 Antibody, clone 12C3
Alternate Names	ras-related protein Rab-25 RAB25, member RAS oncogene family
背景信息	Ras-related protein Rab-25 (Rab25) is a GTP-binding protein belonging to the Rab family of proteins. Rab25 has observed expression in kidney, lung and gastrointestinal mucosa, appearing to be exclusively expressed within epithelial cells. This specific expression has led to the implication that Rab25 has a role as a regulator for vesicular transport associated with epithelia. Rab25 is potentially a selective regulator for transcytotic pathways and apical recycling. Several recent studies have indicated an observable loss of Rab25 in breast cancer tissue and cell lines, and have suggested a potential role for Rab25 as a regulator for Ras induced tumorigenesis.

产品信息
格式	Purified
控制	MCF-7 cell lysate
演示	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide.

应用
应用	Anti-Rab25 Antibody, clone 12C3 is a Mouse Monoclonal Antibody for the detection of Rab25 & has been validated in WB & IHC.
主要应用	Immunohistochemistry Western Blotting
应用说明	Imunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Goldenring, J. R. et al. (1996). Am. J. Physiol. 270(33):G515-G525.) Imunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Casanova, J.E. et al. (1999). Molecular Biology of the Cell. 10:47-61.)

生物信息
免疫原品种	Histidine-tagged recombinant protein corresponding to the C-terminus of rabbit Rab25.
表位	C-terminus
克隆	12C3
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes Rab25 at the C-terminus.
同种型	IgG1κ
品种反应性	RabbitHumanMouseRat
Species Reactivity Note	Demonstrated to react with human. Predicted to react with mouse, rat, and rabbit based on 100% sequence homology.
抗体类型	Monoclonal Antibody
Entrez基因编号	XP_002715439
基因符号	CATX8 RAB25 CATX-8 RAB11C
纯化方法	Protein G
UniProt编号	P46629
UniProt汇总	FUNCTION: May selectively regulate the apical recycling and/or transcytotic pathways. SUBUNIT STRUCTURE: Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4 (By similarity). SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side Potential. TISSUE SPECIFICTY: Expressed in the gastrointestinal mucosa, (highest expression seen in the ileum and colon), kidney, and lung. A very minor and variable level of expression is seen in the splenic tissue. SEQUENCE SIMLARITIES: Belongs to the small GTPase superfamily. Rab family.

产品使用声明
质量保证	Evaluated by Western Blot in MCF-7 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Rab25 on 10 µg of MCF-7 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µg

merck millipore,默克密理博,05-1574,Anti-Rab25 Antibody, clone 12C3

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