merck millipore,默克密理愽
  • merck millipore,默克密理博,05-1406,Anti-Phospholipase A2 Antibody, clone CH-7

  • merck millipore,默克密理博,05-1406,Anti-Phospholipase A2 Antibody, clone CH-7

    产品名称：Anti-Phospholipase A2 Antibody, clone CH-7
    产品型号：05-1406
    Anti-Phospholipase A2 Antibody, clone CH-7 detects level of Phospholipase A2 & has been published & validated for use in WB, ELISA, IH(P).

    merck millipore,默克密理博,05-1406,Anti-Phospholipase A2 Antibody, clone CH-7

  • 产品介绍
  • merck millipore,默克密理博,05-1406,Anti-Phospholipase A2 Antibody, clone CH-7

    Replacement Information
    Replacement Information 05-1406 is a recommended replacement for MAB1668

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MELISA, WBMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-1406
    Replaces MAB1668
    描述 Anti-Phospholipase A2 Antibody, clone CH-7
    Alternate Names
    • Phospholipase A2
    背景信息 Phospholipases hydrolyze phosphoglycerides to form smaller compounds which often serve as critical second messengers. Phospholipase A2 (PLA2) hydrolyzes phosphatidylcholine to produce lysophosphatidylcholine, a second messenger involved in cell proliferation, adhesion, T-cell activation, and smooth muscle cell contractility. PLA2 isoforms include membrane-associated, Ca2+-independent forms, cytosolic, Ca2+-dependent forms, and secretory forms. PLC catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate, to generate inositol (1,4,5) trisphosphate (IP3) and diacylglycerol (DAG), leading to Ca2+ release and PKC activation, respectively. At least four types of PLC have been identified. PLCβ, is activated by G-Protein-Coupled Receptors (GPCRs) and heterotrimeric G-Proteins. Mechanisms of PLCδ activation are unclear, but may also involve GPCRs. PLCγ, is activated by receptor tyrosine kinases, and appears essential for signaling by the PDGF Receptor. PLCε is a Guanine Nucleotide Exchange factor (GEF) for the small G-Protein Ras. PLD activity is up-regulated when cells are stimulated by hormones, growth factors or other extracellular signals. Two distinct PLDs, PLD1 and PLD2, have been identified. PLD hydrolyzes phosphatidylcholine to release phosphatidic acid, which is associated with a number of cellular responses including regulated secretion, mitogenesis and cytoskeletal changes.
    产品信息
    格式 Purified
    控制
    • RAW264 Cell Lysate
    演示 Purified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6.
    应用
    应用 Anti-Phospholipase A2 Antibody, clone CH-7 detects level of Phospholipase A2 & has been published & validated for use in WB, ELISA, IH(P).
    主要应用
    • ELISA
    • Western Blotting
    应用说明 ELISA: A previous lot of this antibody was used in ELISA.
    Optimal working dilutions must be determined by the end user.

    Immunohistochemistry(paraffin): Representative images from a previous lot.
    Optimal Staining of PLA2 Monoclonal Antibody: Colon Cancer

    Western Blot: A previous lot of this antibody was used at 1:500-1:1000; antibody recognizes all isoforms, the cPLA. Alpha (85 kDa) typically migrates as 100-110 kDa on reducing westerns; beta migrates as 114 kDa; antibody will also recognize the sPLA at approximately 14-20 kDa.
    生物信息
    免疫原品种 Recombinant cPLA2
    克隆 CH-7
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes PLA2.
    同种型 IgG2b
    品种反应性 RatHumanMouse
    Species Reactivity Note Human, mouse and rat.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_000928.2
    Entrez基因汇总 Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM]
    基因符号
    • EC 3.1.1.4
    • PLA2G1B
    • MGC119834
    • PPLA2
    • PLA2
    • PLA2A
    • MGC119835
    修改
    • Phosphorylation
    纯化方法 Protein A purfied
    UniProt编号
    • P04054
    UniProt汇总 FUNCTION: SwissProt: P04054 # PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides.
    COFACTOR: Binds 1 calcium ion per subunit (By similarity).
    SIZE: 148 amino acids; 16360 Da
    SUBCELLULAR LOCATION: Secreted.SIMILARITY:SwissProt: P04054 ## Belongs to the phospholipase A2 family.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on RAW 264 lysates.

    Western Blot Analysis: 1:500 dilution of this lot detected PLA2 on 10 μg of RAW 264 lysates.

    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable at 2-8°C for 1 year from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,05-1406,Anti-Phospholipase A2 Antibody, clone CH-7

上一件merck millipore产品：merck millipore,默克密理博,407611,Interleukin-1α, Human, Recombinant, E. coli
下一件merck millipore产品：merck millipore,默克密理博,AP1179,Anti-TLR4 Mouse mAb (1H7)


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