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merck millipore,默克密理博,05-1307,Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2, rabbit monoclonal
产品名称：Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2, rabbit monoclonal
产品型号：05-1307
Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2 is a high quality Rabbit Monoclonal Antibody for the detection of Ubiquitin & has been validated in FC, ICC, IP, IHC, WB, PIA.
merck millipore,默克密理博,05-1307,Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2, rabbit monoclonal
- 产品介绍
- merck millipore,默克密理博,05-1307,Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2, rabbit monoclonal
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rb, H, M, R WB, FC, ICC, IHC Rb Purified Monoclonal Antibody 描述 产品目录编号 05-1307 描述 Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2, rabbit monoclonal 概述 DI-UBIQUITIN PROTEIN AVAILABLE!
Di-Ubiquitin (Lys48-linked) Recombinant Protein (17-10408)背景信息 Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.产品信息 格式 Purified 控制 - Lys48 polyubiquitin chains.
演示 Protein A purified recombinant purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide 应用 应用 Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2 is a high quality Rabbit Monoclonal Antibody for the detection of Ubiquitin & has been validated in FC, ICC, IP, IHC, WB, PIA. 主要应用 - Western Blotting
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry:
Ubiquitin (Lys 48-specific) staining of rat brain sections (left figure) or human breast carcinoma (right figure). Staining pattern detects axons of neurons in brain tissue but not surrounding glial cells, and breast tumor cell cytoplasm is strongly detected without nuclear involvement.
Immunocytochemistry:
Confocal IF analysis of NIH/3T3, HeLa, and A431 cells using anti-Ubiquitin (Lys48 specific) (Red). Actin filaments were labeled with AlexaFluor 488-Phalloidin (Green). Nuclear material is stained with DAPI (Blue).
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys48-specific (#05-1307) (blue) and rabbit IgG control (red).
Immunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-48-specific), clone Apu2, precipitated ubiquitin-containing proteins from HeLa cell lysates.
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys48-specific, with K48-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.生物信息 免疫原品种 Immunogen used was human di-ubiquitin linked at Lys48. 表位 Lys48 ubiquitination site 克隆 Apu2 宿主 Rabbit 特异性 Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin. 同种型 IgG 品种反应性 RabbitHumanMouseRat Species Reactivity Note Human, mouse, rat, rabbit. Based on sequence homology, broad species cross-reactivity is expected. 抗体类型 Monoclonal Antibody 纯化方法 Protein A purfied UniProt编号 - P0CG47
UniProt汇总 FUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ref.21
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
SIMILARITY: Belongs to the ubiquitin family.
产品使用声明 质量保证 Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected Lys-48 ubiquitin chains by Western blotting.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,SLM-021-B,EmbryoMax DMEM (1X), liquid, With 4,500mg/L Glucose, without L-Glutamine and Sodium Pyruvate
下一件merck millipore产品：merck millipore,默克密理博,ABE572,Anti-N6-methyladenosine (m6A) Antibody
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